BBa_K737041
BBa_K737041 Version 1 (Component)
galK::GFP generator ligated to LacI generator and constitutive sRNA device

BBa_K737043
BBa_K737043 Version 1 (Component)
constitutive Spot42 generator ligated to lacI generator

BBa_K737051
BBa_K737051 Version 1 (Component)
IPTG inducible GFP Generator

BBa_K737052
BBa_K737052 Version 1 (Component)
aTc inducible GFP Generaror

BBa_K737059
BBa_K737059 Version 1 (Component)
NanC_Constitutive buffer RNA generator

BBa_K737060
BBa_K737060 Version 1 (Component)
YtfJ_Constitutive buffer RNA generator

BBa_K737061
BBa_K737061 Version 1 (Component)
SrlA_Constitutive buffer RNA generator

BBa_K737062
BBa_K737062 Version 1 (Component)
GalK_Constitutive anti-messenger RNA generator

BBa_K861071
BBa_K861071 Version 1 (Component)
AdrA gene with a RBS and a terminator

BBa_K737066
BBa_K737066 Version 1 (Component)
E0040 without AUG

BBa_K861091
BBa_K861091 Version 1 (Component)
YhjH gene with a RBS and a Terminator

BBa_K863012
BBa_K863012 Version 1 (Component)
tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag

BBa_K737040
BBa_K737040 Version 1 (Component)
galK::GFP generator ligated to TetR generator and constitutive sRNA device

BBa_K737042
BBa_K737042 Version 1 (Component)
constitutive Spot42 generator ligated to TetR generator

BBa_K737053
BBa_K737053 Version 1 (Component)
Plac-GFP Generator ligated to TetR Generator

BBa_K737054
BBa_K737054 Version 1 (Component)
Ptet-GFP Generator ligated to LacI Generator

BBa_K908000
BBa_K908000 Version 1 (Component)
Microcin B17, Gene A

BBa_K908001
BBa_K908001 Version 1 (Component)
Microcin B17, Gene B

BBa_K908002
BBa_K908002 Version 1 (Component)
Microcin B17, Gene C

BBa_K908004
BBa_K908004 Version 1 (Component)
Microcin B17, Gene D

BBa_K908005
BBa_K908005 Version 1 (Component)
Microcin B17, Gene E

BBa_K908006
BBa_K908006 Version 1 (Component)
Microcin B17, Gene F

BBa_K908008
BBa_K908008 Version 1 (Component)
Microcin B17, Gene G

BBa_K908009
BBa_K908009 Version 1 (Component)
Microcin C7, Gene A

BBa_K908010
BBa_K908010 Version 1 (Component)
Microcin C7, Gene B

BBa_K908011
BBa_K908011 Version 1 (Component)
Microcin C7, Gene C

BBa_K908012
BBa_K908012 Version 1 (Component)
Microcin C7, Gene D

BBa_K908013
BBa_K908013 Version 1 (Component)
Microcin C7, Gene E

BBa_K908014
BBa_K908014 Version 1 (Component)
Microcin C7, Gene F

BBa_K908015
BBa_K908015 Version 1 (Component)
Microcin B17, Gene A flanked by rbs and Attc sequence

BBa_K908016
BBa_K908016 Version 1 (Component)
Microcin B17, Gene B flanked by Attc sequence and rbs

BBa_K908017
BBa_K908017 Version 1 (Component)
Microcin B17, Gene C flanked by Attc sequence and rbs

BBa_K908018
BBa_K908018 Version 1 (Component)
Microcin B17, Gene F flanked by Attc sequence and rbs

BBa_K908019
BBa_K908019 Version 1 (Component)
Microcin C7, Gene E flanked by Attc sequence and rbs

BBa_K908020
BBa_K908020 Version 1 (Component)
Microcin C7, Gene C flanked by Attc sequence and rbs

BBa_K908021
BBa_K908021 Version 1 (Component)
Microcin C7, Gene F flanked by Attc sequence and rbs

sboA-AmilC
BBa_K818400 Version 1 (Component)
AmilCP with sboA regulator

BBa_K818500
BBa_K818500 Version 1 (Component)
Lycopene with sboA regulator

sboA-AmilG
BBa_K818600 Version 1 (Component)
AmilGFP with sboA regulator

BBa_K863020
BBa_K863020 Version 1 (Component)
bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag

BM-CFP
BBa_K774004 Version 1 (Component)
Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420

BM-RFP
BBa_K774005 Version 1 (Component)
Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B

MB-CFP
BBa_K774006 Version 1 (Component)
Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420

MB-RFP
BBa_K774007 Version 1 (Component)
Mammalian-Bacterial promoter with RFP reporter: CArG promoter sequence + BBaK216005 + BBa_K08101

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

T7Gene6
BBa_K093001 Version 1 (Component)
T7 Gene 6 exonuclease

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.