BBa_K1115010
BBa_K1115010 Version 1 (Component)
Constitutive production (PTet) of dhlB and dhlA

BBa_K1491023
BBa_K1491023 Version 1 (Component)
C-terminus of T7 RNA Polymerase

STAR
BBa_K1893013 Version 1 (Component)
Small transcription activating RNA (STAR)

BBa_K115009
BBa_K115009 Version 1 (Component)
RNA thermometer (FourU)

BBa_K1906012
BBa_K1906012 Version 1 (Component)
RNAT Mut

BBa_J25900
BBa_J25900 Version 1 (Component)
pSB1A3 with readthrough protection

BBa_K1806004
BBa_K1806004 Version 1 (Component)
T7+ ibPB RNA Thermometer + pelB + MCS + 6xHis

BTE genera
BBa_K558007 Version 1 (Component)
Bay Laurel Thioesterse Generator for Fatty Acid Production

BBa_J24008
BBa_J24008 Version 1 (Component)
pLasR promoter with YFP and cI production

BBa_K1448013
BBa_K1448013 Version 1 (Component)
In E coli mono-terpene production cassette

BBa_J100108
BBa_J100108 Version 1 (Component)
Riboswitch and GFP +High production of eCDM8

BBa_K137118
BBa_K137118 Version 1 (Component)
AHL induced production of rcsA; medium strength

BBa_K729009
BBa_K729009 Version 1 (Component)
Three fold active biological containment System

SspDnaB(N)
BBa_K1362408 Version 1 (Component)
SspDnaB N-Intein cloning piece

BBa_Y1011
BBa_Y1011 Version 1 (Component)
Act1 Promoter

BBa_K1859012
BBa_K1859012 Version 1 (Component)
2*GSGSGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859010
BBa_K1859010 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*3 linker

BBa_K1859009
BBa_K1859009 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS*3 linker

BBa_K1859014
BBa_K1859014 Version 1 (Component)
3*GSGSGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859007
BBa_K1859007 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS*2 linker

BBa_K1859011
BBa_K1859011 Version 1 (Component)
2*GGSGGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859008
BBa_K1859008 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*2 linker

BBa_K1859013
BBa_K1859013 Version 1 (Component)
3*GGSGGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1065305
BBa_K1065305 Version 1 (Component)
Blue light sensor without inverter for the production of amilGFP

BBa_M1357
BBa_M1357 Version 1 (Component)
1-butanol production pathway in integration plasmid

BBa_M1675
BBa_M1675 Version 1 (Component)
ACTN3 gene [Sam Hawkins]

BBa_K737058
BBa_K737058 Version 1 (Component)
Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose

C6
BBa_K1366105 Version 1 (Component)
Production of survivin siRNA under the hTERT promoter (C6)

C7
BBa_K1366106 Version 1 (Component)
Production of survivin siRNA under a hTERT promoter (C7)

C8
BBa_K1366107 Version 1 (Component)
Production of survivin siRNA under an hTERT promoter (C8)

BBa_K895007
BBa_K895007 Version 1 (Component)
Tetrathionate-sensing two-component system linked to GFP production.

BBa_K1197015
BBa_K1197015 Version 1 (Component)
Catalytically active CYP6G1 coding device

CircNpu
BBa_K1362000 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct

BBa_K1792000
BBa_K1792000 Version 1 (Component)
Petal Death Protein (PDP) - Oxalic acid production

BBa_K1407010
BBa_K1407010 Version 1 (Component)
constitutive production of LasR+LasR/PAI1 Inducible promoter +RBS(B0030)+RFP+Terminator

BBa_K127999
BBa_K127999 Version 1 (Component)
Saito Cell for self reproduction

BBa_K587005
BBa_K587005 Version 1 (Component)
iTast -> inhibits the action of tast

BBa_K117009
BBa_K117009 Version 1 (Component)
colicin E7 production system induced by lactose

BBa_K1903019
BBa_K1903019 Version 1 (Component)
Intein N SSp DnaB S1

BBa_K1020011
BBa_K1020011 Version 1 (Component)
High expressed Alkane Selector for intracellular alkane production

BBa_K1653026
BBa_K1653026 Version 1 (Component)
Geranyl diphosphate (geraniol precursor）production. dev

BBa_K2109002
BBa_K2109002 Version 1 (Component)
Nanobody - RNAp Fusion

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

BBa_M41017
BBa_M41017 Version 1 (Component)
This sequence encodes a 64-nucleotide RNAi.

BBa_K1994014
BBa_K1994014 Version 1 (Component)
Sigma-54 E. coli RNAP subunit

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

BBa_K283047
BBa_K283047 Version 1 (Component)
T7 RNA polymerase

BBa_K1065310
BBa_K1065310 Version 1 (Component)
Blue light circuit with inverter for the production of amilCP

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1060013
BBa_K1060013 Version 1 (Component)
LacI-AroG* production under regulation of TetR repressible promoter