BBa_J72198
BBa_J72198 Version 1 (Component)
Payload Delivery Device with Barnase Payload

BBa_J72208
BBa_J72208 Version 1 (Component)
Kanamycin resistant BAC with R6K origin

BBa_J72210
BBa_J72210 Version 1 (Component)
Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site

BBa_J72211
BBa_J72211 Version 1 (Component)
ampicillin resistant p15A vector with dapD

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K812001
BBa_K812001 Version 1 (Component)
Biobricked pSC2+ plasmid with pElastase promoter for frog use

GFP-AID
BBa_K812010 Version 1 (Component)
GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)

BBa_K817017
BBa_K817017 Version 1 (Component)
pSB2K3 with parS site

BBa_K737011
BBa_K737011 Version 1 (Component)
gvpC-GFP fusion protein with medium constitutive promoter

BBa_K737021
BBa_K737021 Version 1 (Component)
gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K737012
BBa_K737012 Version 1 (Component)
gvpC-GFP fusion protein UH with medium constitutive promoter

BBa_K737022
BBa_K737022 Version 1 (Component)
This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K863005
BBa_K863005 Version 1 (Component)
ecol laccase from E. coli with T7 promoter, RBS and His-tag

BBa_K863000
BBa_K863000 Version 1 (Component)
bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag

BBa_K863010
BBa_K863010 Version 1 (Component)
tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag

BBa_K861071
BBa_K861071 Version 1 (Component)
AdrA gene with a RBS and a terminator

BBa_K737066
BBa_K737066 Version 1 (Component)
E0040 without AUG

BBa_K861091
BBa_K861091 Version 1 (Component)
YhjH gene with a RBS and a Terminator

BBa_K863012
BBa_K863012 Version 1 (Component)
tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag

sboA-AmilC
BBa_K818400 Version 1 (Component)
AmilCP with sboA regulator

BBa_K818500
BBa_K818500 Version 1 (Component)
Lycopene with sboA regulator

sboA-AmilG
BBa_K818600 Version 1 (Component)
AmilGFP with sboA regulator

BBa_K863020
BBa_K863020 Version 1 (Component)
bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag

BM-CFP
BBa_K774004 Version 1 (Component)
Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420

BM-RFP
BBa_K774005 Version 1 (Component)
Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B

MB-CFP
BBa_K774006 Version 1 (Component)
Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420

MB-RFP
BBa_K774007 Version 1 (Component)
Mammalian-Bacterial promoter with RFP reporter: CArG promoter sequence + BBaK216005 + BBa_K08101

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K098999
BBa_K098999 Version 1 (Component)
mtrB with RBS

BBa_K098998
BBa_K098998 Version 1 (Component)
cIts coding region with rbs

BBa_K098996
BBa_K098996 Version 1 (Component)
mtrB with terminator

BBa_K098994
BBa_K098994 Version 1 (Component)
mtrB with RBS and terminator

BBa_K098995
BBa_K098995 Version 1 (Component)
heat sensitive cI QPI with high promoter

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K143071
BBa_K143071 Version 1 (Component)
AmyE integratable PoPS generator (P43-gsiB) (with CmR)

BBa_K143072
BBa_K143072 Version 1 (Component)
AmyE integratable PoPS generator (P43-spoVG) (with CmR)

BBa_K143073
BBa_K143073 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)

BBa_K143074
BBa_K143074 Version 1 (Component)
AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)