BBa_K1973019
BBa_K1973019 Version 1 (Component)
miniTn7BB-Gm-nahR-Psal-nasF

BBa_K1706010
BBa_K1706010 Version 1 (Component)
T7-PADH-NAD

BBa_K1688012
BBa_K1688012 Version 1 (Component)
dTomato (inc RBS, NahR/Psal promoter system)

BBa_K1973023
BBa_K1973023 Version 1 (Component)
miniTn7BB-Gm-nahR-Psal-pleD*

BBa_K1973024
BBa_K1973024 Version 1 (Component)
miniTn7BB-Gm-nahR-Psal-xylS2

BBa_K1973022
BBa_K1973022 Version 1 (Component)
miniTn7BB-Gm-nahR-Psal-yhjH

BBa_K1973032
BBa_K1973032 Version 1 (Component)
miniTn7BBGm-nahR-Psal-nasF-epi

BBa_K1973028
BBa_K1973028 Version 1 (Component)
miniTn7BBGm-nahR-Psal-nasF-glpF

BBa_K1973038
BBa_K1973038 Version 1 (Component)
miniTn7BBGm-nahR-Psal-pleD*-glpF

BBa_K957015
BBa_K957015 Version 1 (Component)
Naphthalene Dioxygenase (From Pseudomonas NAH7 plasmid)

BBa_K1031019
BBa_K1031019 Version 1 (Component)
NahR-Psal-B0034-RiboJ-φR73δ

BBa_K957004
BBa_K957004 Version 1 (Component)
nah Operon for degradation of naphthalene to salicylate

BBa_K1973009
BBa_K1973009 Version 1 (Component)
miniTn7BB-Gm-nahR-Psal-nasF-lapG

BBa_K1973012
BBa_K1973012 Version 1 (Component)
miniTn7BB-Gm-nahR-Psal-nasF-pleD*

BBa_K1973010
BBa_K1973010 Version 1 (Component)
miniTn7BB-Gm-nahR-Psal-nasF-yhjH

BBa_K1973021
BBa_K1973021 Version 1 (Component)
miniTn7BB-Gm-nahR-Psal-nasF-xylS2

BBa_K1619001
BBa_K1619001 Version 1 (Component)
medh2 (NAD+ dependent methanol dehydrogenase)

BBa_K1688015
BBa_K1688015 Version 1 (Component)
Nah7 - Upper naphthalene breakdown pathway (inc RBS)

ndh-lva
BBa_K1036001 Version 1 (Component)
the ndh gene coding for respiratory NADH dehydrogenase II

BBa_M11008
BBa_M11008 Version 1 (Component)
NADH-dependent butanol dehydrogenase B (bdhB/BDH II)

BBa_M11007
BBa_M11007 Version 1 (Component)
NADH-dependent butanol dehydrogenase A (bdhA/BDH I)

BBa_K1688017
BBa_K1688017 Version 1 (Component)
Nah7 - Mutagenized upper naphthalene breakdown pathway (inc RBS)

BBa_K235028
BBa_K235028 Version 1 (Component)
[K235024][K235001] (NAND gate control test, positive control)

BBa_K1523021
BBa_K1523021 Version 1 (Component)
nahR coding sequence,encoding a LysR-type transcriptional regulator

BBa_K235026
BBa_K235026 Version 1 (Component)
[K235022][K235001] (NAND gate control test, pLac positive control)

BBa_K235027
BBa_K235027 Version 1 (Component)
[K235024][K235000] (NAND gate control test, arabinose positive control)

BBa_K1688014
BBa_K1688014 Version 1 (Component)
Nah7 - Upper naphthalene breakdown pathway (inc RBS, J23101 promoter)

BBa_K1688013
BBa_K1688013 Version 1 (Component)
Nah7 - Upper naphthalene breakdown pathway (inc RBS, J23110 promoter)

BBa_K1688005
BBa_K1688005 Version 1 (Component)
dTomato and ModLac with HlyA tag (inc RBS, NahR/Psal promoter system)

BBa_K1688009
BBa_K1688009 Version 1 (Component)
dTomato, ModLac and Dioxygenase (inc RBS, NahR/Psal promoter system)

BBa_K957006
BBa_K957006 Version 1 (Component)
Constitutive expression of nah Operon with oriT

BBa_K581017
BBa_K581017 Version 1 (Component)
araC-pBAD-supD-nahR-psal-supD-pT7-rbs-gfp

BBa_K957005
BBa_K957005 Version 1 (Component)
Constitutive expression of nah Operon for degradation of naphthalene to salicylate

BBa_K1716002
BBa_K1716002 Version 1 (Component)
NahR Biosensor for detection of acetylsalicylic acid/aspirin with blue chromoprotein reporter

BBa_K235025
BBa_K235025 Version 1 (Component)
[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

BBa_K896599
BBa_K896599 Version 1 (Component)
Strophurus rankini voucher AMS R140490 NADH dehydrogenase subunit 4 (ND4) gene, partial cds; tRNA-Hi

luxR-ndh
BBa_K1036000 Version 1 (Component)
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.