BBa_K658003
BBa_K658003 Version 1 (Component)
a bacteria population-control device with RBS0.6 driven by lacl+pL

BBa_K1615020
BBa_K1615020 Version 1 (Component)
Linker-CBDcipA-linker fused to MorA driven by LacI promoter

BBa_K1615109
BBa_K1615109 Version 1 (Component)
Linker-CBDcipA-linker fused to mRFP driven by LacI promoter

BBa_K1615059
BBa_K1615059 Version 1 (Component)
Linker+dCBD fused to Heroin Esterase driven by LacI promoter

BBa_K1615043
BBa_K1615043 Version 1 (Component)
MaoA fused to Linker-CBDcipA-linker driven by LacI promoter

BBa_K1615110
BBa_K1615110 Version 1 (Component)
mRFP fused to Linker-CBDcipA-linker driven by LacI promoter

BBa_K1615021
BBa_K1615021 Version 1 (Component)
MorA fused to Linker-CBDcipA-linker driven by LacI promoter

BBa_K1615042
BBa_K1615042 Version 1 (Component)
Linker-CBDcipA-linker fused to MaoA driven by LacI promoter

BBa_K1615057
BBa_K1615057 Version 1 (Component)
CBDcenA+Linker fused to Heroin Esterase driven by LacI promoter

BBa_K1321295
BBa_K1321295 Version 1 (Component)
CBDclos fused to sfGFP driven by LacI in pSEVA331-Bb

BBa_K1321296
BBa_K1321296 Version 1 (Component)
sfGFP fused to CBDclos driven by LacI in pSEVA331-Bb

BBa_K1321297
BBa_K1321297 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI in pSEVA331-Bb

BBa_K2082001
BBa_K2082001 Version 1 (Component)
Nanobody: Constant regions (insert variable regions to create your defined Nanobody or library)

BBa_K1848006
BBa_K1848006 Version 1 (Component)
LuxR/AHL driven Lysis Cassette with Protein level Timer Delay

BBa_K1368008
BBa_K1368008 Version 1 (Component)
mcherry generator driven by HSL under the post transcriptional control

BBa_K1428000
BBa_K1428000 Version 1 (Component)
T7 promoter-driven BMC structural genes with a His6-tagged component for purification

BBa_K1615065
BBa_K1615065 Version 1 (Component)
Linker-CBDcipA-linker fused to Heroin Esterase driven by LacI promoter

BBa_K1615013
BBa_K1615013 Version 1 (Component)
MorA fused to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K1615035
BBa_K1615035 Version 1 (Component)
MaoA fused to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K1615102
BBa_K1615102 Version 1 (Component)
mRFP used to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K1615066
BBa_K1615066 Version 1 (Component)
Heroin Esterase fused to Linker-CBDcipA-linker driven by LacI promoter

BBa_K1615080
BBa_K1615080 Version 1 (Component)
TVEL5 fused to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K726007
BBa_K726007 Version 1 (Component)
T7 driven lac operated inducer for the las quorum-sensing system

BBa_K1615088
BBa_K1615088 Version 1 (Component)
TVEL5 laccase fused to Linker-CBDcipA-linker driven by LacI promoter

BBa_K2066112
BBa_K2066112 Version 1 (Component)
Synthetic Enhancer Project: Ntr promoter driven NRII2302 (mut) on UNS Standard

iccdB1.0
BBa_K658001 Version 1 (Component)
a bacteria population-control device with RBS1.0 driven by lacl+pL

Plac/ara-1
BBa_K1145006 Version 1 (Component)
Drive the expression of LuxRI in pLuxRI2 and its derivatives.

BBa_K658002
BBa_K658002 Version 1 (Component)
a bacteria population-control device with RBS0.6 driven by lacl+pL

iccdB0.07
BBa_K658005 Version 1 (Component)
a bacteria population-control device with RBS0.07 driven by lacl+pL

BBa_K658004
BBa_K658004 Version 1 (Component)
a bacteria population-control device with RBS0.3 driven by lacl+pL

BBa_K1615058
BBa_K1615058 Version 1 (Component)
Heroin esterase fused to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K658014
BBa_K658014 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-5 Driven by PLlac 0-1

BBa_K658013
BBa_K658013 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-3 Driven by PLlac 0-1

BBa_K1412010
BBa_K1412010 Version 1 (Component)
Drive the E.coli engineered strain(CL-1) towards death region

BBa_K1412008
BBa_K1412008 Version 1 (Component)
Drive the E.coli engineered strain(CL-1) towards death region

BBa_K658015
BBa_K658015 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-3/5 Driven by PLlac 0-1

BBa_K658010
BBa_K658010 Version 1 (Component)
a bacteria population-control device with lux pR-5 driven by lacl+pL

BBa_K658009
BBa_K658009 Version 1 (Component)
a bacteria population-control device with lux pR-3 driven by lacl+pL

BBa_K658011
BBa_K658011 Version 1 (Component)
a bacteria population-control device with lux pR-3/5 driven by lacl+pL

iGEM 2019 Plates
iGEM_2019_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2019

iGEM 2018 Plates
iGEM_2018_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2018

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.