BBa_K1022129
BBa_K1022129 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II:RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly

BBa_K1022128
BBa_K1022128 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly

BBa_K1022127
BBa_K1022127 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lys

BBa_M1530
BBa_M1530 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

BBa_M1515
BBa_M1515 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1529
BBa_M1529 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1541
BBa_M1541 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1531
BBa_M1531 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1542
BBa_M1542 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1535
BBa_M1535 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1536
BBa_M1536 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

BBa_J70609
BBa_J70609 Version 1 (Component)
RFC12 7x His Tag Head Domain

BBa_K1669000
BBa_K1669000 Version 1 (Component)
CtfA + His-tag

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_B0056
BBa_B0056 Version 1 (Component)
E. coli his operon terminator

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_I759037
BBa_I759037 Version 1 (Component)
cis3-repressed, tet-regulated YFP

BBa_J70621
BBa_J70621 Version 1 (Component)
RFC12 7x His Tag Tail Domain

BBa_J70560
BBa_J70560 Version 1 (Component)
GFP, with N-terminal 6 his

BBa_K1362300
BBa_K1362300 Version 1 (Component)
NpuDnaE N-Intein RFC[105] assembly construct (with His6)

BBa_K1363200
BBa_K1363200 Version 1 (Component)
Anti-LPS factor(LALF) regulated by lacI

BBa_K1732010
BBa_K1732010 Version 1 (Component)
pSB1C3-J23100-B0034-E0040-His-B0015

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K563004
BBa_K563004 Version 1 (Component)
pTEF1, promoter of Translational elongation factor EF-1 alpha

BBa_K1974011
BBa_K1974011 Version 1 (Component)
T7 Promoter+RBS+Hv1a+linker+6X His-Tag

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

BBa_K1989037
BBa_K1989037 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PhoA

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K1974022
BBa_K1974022 Version 1 (Component)
T7Promoter+RBS+Sf1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974021
BBa_K1974021 Version 1 (Component)
T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974023
BBa_K1974023 Version 1 (Component)
T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.