Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K390001
BBa_K390001 Version 1 (Component)
5'UTR and native RBS from psbA2 in Synechocystis sp. PCC 6803

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_K390002
BBa_K390002 Version 1 (Component)
5'UTR and consensus RBS from psbA2 in Synechocystis sp. PCC 6803

BBa_I15008
BBa_I15008 Version 1 (Component)
heme oxygenase (ho1) from Synechocystis

pSB1A2
pSB1A2 Version 1 (Component)
pSB1A2 (Replaced by pSB1A3)

BBa_I15009
BBa_I15009 Version 1 (Component)
phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K390005
BBa_K390005 Version 1 (Component)
psbA2 Type I promoter from Synechocystis sp. PCC 6803

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K390007
BBa_K390007 Version 1 (Component)
sigE Type II promoter from Synechocystis sp. PCC 6803

BBa_K390011
BBa_K390011 Version 1 (Component)
glnBP2 Type II promoter from Synechocystis sp. PCC 6803

BBa_K390012
BBa_K390012 Version 1 (Component)
pilA1 Type III promoter from Synechocystis sp. PCC 6803

BBa_K390010
BBa_K390010 Version 1 (Component)
hspA Type I Promoter from Synechocystis sp. PCC 6803

BBa_K390008
BBa_K390008 Version 1 (Component)
lrtA Type II promoter from Synechocystis sp. PCC 6803

BBa_K390009
BBa_K390009 Version 1 (Component)
sigF Type III promoter from Synechocystis sp. PCC 6803

BBa_K390006
BBa_K390006 Version 1 (Component)
sigA Type I promoter from Synechocystis sp. PCC 6803

BBa_K1476001
BBa_K1476001 Version 1 (Component)
PilA1 from Synechocystis sp. PCC 6803

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

BBa_K1476004
BBa_K1476004 Version 1 (Component)
petF from Synechocystis sp. PCC 6803

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS