Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

OmpR
BBa_R0082 Version 1 (Component)
Promoter (OmpR, positive)

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

GFP genera
BBa_E0840 Version 1 (Component)
GFP generator

GFP report
BBa_E0240 Version 1 (Component)
GFP generator

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

SEGA021
SEGA021 Version 1 (Component)
natural linear DNA.

BBa_K215200
BBa_K215200 Version 1 (Component)
Generic Surface Display Construct: IPTG Inducible Lpp + 1tmr OmpA + NheI Site

SEGA022
SEGA022 Version 1 (Component)
natural linear DNA.

OmpA
BBa_K103006 Version 1 (Component)
OmpA outer membrane protein A fused to linker; displays proteins on cell surface

BBa_K215201
BBa_K215201 Version 1 (Component)
Generic Surface Display Construct: IPTG Inducible Lpp + 5tmr OmpA + NheI Site

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_J61100
BBa_J61100 Version 1 (Component)
Ribosome Binding Site Family Member

OmpR
BBa_R0084 Version 1 (Component)
Promoter (OmpR, positive)

BBa_J85600
BBa_J85600 Version 1 (Component)
attB1 recombination site

BBa_K215250
BBa_K215250 Version 1 (Component)
Lpp + 1tmr OmpA + GS Linker + TEV site + NheI Site

BBa_K215210
BBa_K215210 Version 1 (Component)
IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin

BBa_K215211
BBa_K215211 Version 1 (Component)
IPTG Inducible 5tmr OmpA Surface Display of WT Streptavidin

BBa_K215251
BBa_K215251 Version 1 (Component)
Lpp + 5tmr OmpA + GS Linker + TEV site + NheI Site

OmpR
BBa_R0083 Version 1 (Component)
Promoter (OmpR, positive)

spacer
BBa_B0040 Version 1 (Component)
Spacer.1 (generic)

Ara
BBa_K808000 Version 1 (Component)
araC-Pbad - Arabinose inducible regulatory promoter/repressor unit

Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

BBa_K1694002
BBa_K1694002 Version 1 (Component)
Lpp-OmpA-N

BBa_K215260
BBa_K215260 Version 1 (Component)
Lpp + 1tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His

BBa_K215261
BBa_K215261 Version 1 (Component)
Lpp + 5tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His

BBa_I13401
BBa_I13401 Version 1 (Component)
GFP reporter for RHS of library test constructs

BBa_K346034
BBa_K346034 Version 1 (Component)
T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of mercury binding peptide)

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

BBa_K346029
BBa_K346029 Version 1 (Component)
T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of lead binding peptide)

scar
BBa_G0004 Version 1 (Component)
SpeI/NheI mixed site

scar
BBa_G0003 Version 1 (Component)
NheI/XbaI mixed site

BBa_K1921017
BBa_K1921017 Version 1 (Component)
LPP-OmpA

BBa_K914003
BBa_K914003 Version 1 (Component)
L-rhamnose-inducible promoter (pRha)

BBa_J36848
BBa_J36848 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin wild-type + His6tag

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

BBa_K195401
BBa_K195401 Version 1 (Component)
Lpp-OmpA-linker -F10scFv