Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

GFP report
BBa_E0240 Version 1 (Component)
GFP generator

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

GFP genera
BBa_E0840 Version 1 (Component)
GFP generator

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

BBa_B0025
BBa_B0025 Version 1 (Component)
double terminator (B0015), reversed

pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter

spacer
BBa_B0040 Version 1 (Component)
Spacer.1 (generic)

AraC
BBa_R0080 Version 1 (Component)
Promoter (AraC regulated)

BBa_B0016
BBa_B0016 Version 1 (Component)
Terminator (T7 RNAP specific, T_Phi)

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_J23100
BBa_J23100 Version 1 (Component)
constitutive promoter family member

BBa_J23106
BBa_J23106 Version 1 (Component)
constitutive promoter family member

BBa_I13453
BBa_I13453 Version 1 (Component)
Pbad promoter

pT7
BBa_I719005 Version 1 (Component)
T7 Promoter

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

BBa_B0024
BBa_B0024 Version 1 (Component)
double terminator (B0012-B0011), reversed

cI HK022
BBa_C0050 Version 1 (Component)
cI repressor from phage HK022 (+LVA?)

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

BBa_J23119
BBa_J23119 Version 1 (Component)
constitutive promoter family member

BBa_B0017
BBa_B0017 Version 1 (Component)
double terminator (B0010-B0010)

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_B0035
BBa_B0035 Version 1 (Component)
RBS (B0030 derivative)

BBa_E0044
BBa_E0044 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)

lux pR
BBa_R0062 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pR)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

TOPO
BBa_B0044 Version 1 (Component)
Topoisomerase I mediated cloning site (reverse)

TOPO
BBa_B0043 Version 1 (Component)
Topoisomerase I mediated cloning site (forward)

BBa_K1847014
BBa_K1847014 Version 1 (Component)
placUV5-lacO

BBa_J23101
BBa_J23101 Version 1 (Component)
constitutive promoter family member

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ