Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

URA5
GenBank_CM000046_Region_302227_303005 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

URA5
GenBank_CM000046_Region_301512_302226 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

URA5
GenBank_CM000046_Region_303006_303540 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

BBa_I13453
BBa_I13453 Version 1 (Component)
Pbad promoter

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

BBa_J23100
BBa_J23100 Version 1 (Component)
constitutive promoter family member

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

pBAD
BBa_K206000 Version 1 (Component)
pBAD strong

scar
BBa_J176041 Version 1 (Component)
Silver scar

lux pR
BBa_R0062 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pR)

BBa_J23119
BBa_J23119 Version 1 (Component)
constitutive promoter family member

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

BBa_J23101
BBa_J23101 Version 1 (Component)
constitutive promoter family member

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

BBa_K187367
BBa_K187367 Version 1 (Component)
pBA_F (B end) USER primer for BioBytes plasmid pBA

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device