BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

AraC
BBa_R0080 Version 1 (Component)
Promoter (AraC regulated)

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

BBa_B0016
BBa_B0016 Version 1 (Component)
Terminator (T7 RNAP specific, T_Phi)

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

cI HK022
BBa_C0050 Version 1 (Component)
cI repressor from phage HK022 (+LVA?)

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

BBa_B0017
BBa_B0017 Version 1 (Component)
double terminator (B0010-B0010)

spacer
BBa_B0040 Version 1 (Component)
Spacer.1 (generic)

tetA(C)b
BBa_J31006 Version 1 (Component)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

cI HK022
BBa_R0050 Version 1 (Component)
Promoter (HK022 cI regulated)

BBa_J64101
BBa_J64101 Version 1 (Component)
ColE1 biobrick plasmid with backward terminator

BBa_J31013
BBa_J31013 Version 1 (Component)
pLac Backwards [cf. BBa_R0010]

BBa_B0013
BBa_B0013 Version 1 (Component)
TE from coliphage T7 (+/-)

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_B1010
BBa_B1010 Version 1 (Component)
Terninator (artificial, large, %T~<10)

BBa_K864400
BBa_K864400 Version 1 (Component)
Ptac, trp & lac regulated promoter

lacI
BBa_C0013 Version 1 (Component)
Repressor, lacI

VF2
BBa_G00100 Version 1 (Component)
Forward primer for sequencing/amplifying BioBrick parts (VF2)

BBa_K208010
BBa_K208010 Version 1 (Component)
Lac Promoter (BBa_R0010) and RBS (B0034)

BBa_K133016
BBa_K133016 Version 1 (Component)
CF

cheY
BBa_C0020 Version 1 (Component)
CheY "tumbling" chemotaxis coding sequence

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

dsRed
BBa_E0010 Version 1 (Component)
dsRed (LVA-)

CAT
BBa_C0100 Version 1 (Component)
Enzyme, chloramphenicol acetyltransferase or CAT

pLac
BBa_K1638000 Version 1 (Component)
Lac operator

scar
BBa_G0001 Version 1 (Component)
XbaI/SpeI mixed site, reverse

BBa_B0070
BBa_B0070 Version 1 (Component)
Specialized RBS modified from that of B0036 (Brink et al.)

OmpA 5
BBa_B0100 Version 1 (Component)
OmpA 5