Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K1583100
BBa_K1583100 Version 1 (Component)
pRha + CsgA

BBa_K914003
BBa_K914003 Version 1 (Component)
L-rhamnose-inducible promoter (pRha)

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_K1583101
BBa_K1583101 Version 1 (Component)
pRha + CsgA + His-tag

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

PEST
BBa_J176018 Version 1 (Component)
PEST

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_K1583107
BBa_K1583107 Version 1 (Component)
pRha + CsgA with His-tag & pTet + RFP

BBa_K1583102
BBa_K1583102 Version 1 (Component)
pRha + CsgA + Hydroxyapatite-affinity tag

BBa_K1583112
BBa_K1583112 Version 1 (Component)
pRha + CsgA & GFP in same operon

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

BBa_K1223006
BBa_K1223006 Version 1 (Component)
His-Tag

BBa_K115003
BBa_K115003 Version 1 (Component)
RNA thermometer (PrfA)

BBa_K1404007
BBa_K1404007 Version 1 (Component)
p70-CsgA-His, His-tagged curli generator

BBa_K1316015
BBa_K1316015 Version 1 (Component)
pRhamnose regulating csgB and csgA curli proteins

Hin
BBa_J31000 Version 1 (Component)
DNA-invertase Hin from Salmonella typhimurium

BBa_K1361998
BBa_K1361998 Version 1 (Component)
Curli Fiber assembly associated protein CsgA, CsgC

BBa_K1404008
BBa_K1404008 Version 1 (Component)
p70-CsgA-His*2, double His-tagged curli generator

BBa_K1361994
BBa_K1361994 Version 1 (Component)
Curli Fiber major monomer-CsgA with his tag

BBa_K1583108
BBa_K1583108 Version 1 (Component)
pRha + CsgA with Hydroxyapatite-affinity tag & pTet + RFP

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

BBa_K1583106
BBa_K1583106 Version 1 (Component)
pRha + CsgA & pTet + RFP

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

pRha
BBa_K902065 Version 1 (Component)
Rhamnose inducible, glucose repressible promoter (pRha)

BBa_K726003
BBa_K726003 Version 1 (Component)
T7 promoter + lac operator + His tag

BBa_K416003
BBa_K416003 Version 1 (Component)
Yeast Secretion Tag

RBS-GFP-Te
BBa_I763020 Version 1 (Component)
RBS-GFP (+LVA Tag)-Terminators

BBa_K1361992
BBa_K1361992 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber

BBa_K1583204
BBa_K1583204 Version 1 (Component)
Medium constitutive promoter + CsgB & CsgC

BBa_K1861102
BBa_K1861102 Version 1 (Component)
His-Tag

BBa_J70042
BBa_J70042 Version 1 (Component)
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)

BBa_K1375024
BBa_K1375024 Version 1 (Component)
Prhl/las +RBS

BBa_B0053
BBa_B0053 Version 1 (Component)
Terminator (His)

polyA
BBa_J176009 Version 1 (Component)
polyA2