BBa_M50051
BBa_M50051 Version 1 (Component)
IPTG Inducible Plasmid for the Expression of LsrR Repressor

BBa_K218021
BBa_K218021 Version 1 (Component)
LuxO D47A under constitutive expression of tetR repressible promoter

BBa_K1472611
BBa_K1472611 Version 1 (Component)
IPTG inducible expression of ∆9, ∆12, and ∆15 desaturases

BBa_K101004
BBa_K101004 Version 1 (Component)
Construct of LacI promoter, RBS, p22 mnt gene, terminator

BBa_K2039000
BBa_K2039000 Version 1 (Component)
Part expressing the dimerization protein FKBP and GFP10 (subunit of tripartite GFP)

BBa_K2039001
BBa_K2039001 Version 1 (Component)
Part expressing the dimerization protein FRB* and GFP11 (subunit of tripartite GFP)

CBDclos(T7
BBa_K863112 Version 1 (Component)
Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K748000
BBa_K748000 Version 1 (Component)
AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.

BBa_K524203
BBa_K524203 Version 1 (Component)
pSC101 heat sensitive origin of replication with deletion in par locus

BBa_K1412089
BBa_K1412089 Version 1 (Component)
Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit

BBa_K1790001
BBa_K1790001 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790005
BBa_K1790005 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790000
BBa_K1790000 Version 1 (Component)
biosensors detect food allergens. A proteins conformation change in response to ligand binding coupl

BBa_K1790003
BBa_K1790003 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790004
BBa_K1790004 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

TALEA-dife
BBa_K1189021 Version 1 (Component)
TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker

BBa_K1790002
BBa_K1790002 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1606014
BBa_K1606014 Version 1 (Component)
Strong promoter without thickener of LuxR and antiholin sequence

BBa_I711016
BBa_I711016 Version 1 (Component)
Butyric Acid -> Butyrl-CoA (constitutive prod. of ctfA, ctfB)

BBa_K656007
BBa_K656007 Version 1 (Component)
Cyan fluorescent protein bound to dockerin of C. thermocellum

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1933103
BBa_K1933103 Version 1 (Component)
constitutive expression of CBDclos fused to BclA with 6xHis tag

BBa_K1933100
BBa_K1933100 Version 1 (Component)
constitutive expression of CBDcex fused to INPNC with 6xHis tag

BBa_K1933102
BBa_K1933102 Version 1 (Component)
constitutive expression of CBDcex fused to BclA with 6xHis tag

BBa_K1933101
BBa_K1933101 Version 1 (Component)
constitutive expression of CBDclos fused to INPNC with 6xHis tag

BBa_Y00029
BBa_Y00029 Version 1 (Component)
S. pombe homolog of S.cerevisiae SGF29, recoded for expression in S.c.

BBa_I13211
BBa_I13211 Version 1 (Component)
Biobricked version of the natural Lux quorum sensing system

BBa_J95005
BBa_J95005 Version 1 (Component)
One of two Bioluminescence based BioBrick RBS characterization kits

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_M31538
BBa_M31538 Version 1 (Component)
This is the trio of genes in M13K07 responsible for Genome Amplification

BBa_K1758102
BBa_K1758102 Version 1 (Component)
Translation enhancing 5-UTR + sfGFP under control of T7 promoter

BBa_K2117011
BBa_K2117011 Version 1 (Component)
ECFP under regulation of the Y. lipolytica native promoter TEF1

BBa_K2018048
BBa_K2018048 Version 1 (Component)
BioBrick that produces large amounts of plastic and secretes it

BBa_K395163
BBa_K395163 Version 1 (Component)
KanR activated by RhlR and C4HSL(AHL of Rhl family)

BBa_K1935015
BBa_K1935015 Version 1 (Component)
NLP-22 under control of FRQ promoter (pFRQ::NLP-22)

BBa_K199070
BBa_K199070 Version 1 (Component)
I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC

BBa_I8030
BBa_I8030 Version 1 (Component)
Booperator aiia test: degradation of HSL "B" by aiia

BBa_K091209
BBa_K091209 Version 1 (Component)
lacIQ promoter under the control of the represseor LacI

BBa_K1961004
BBa_K1961004 Version 1 (Component)
PbrR generator expressed by GFP under autoregulation of PTet

BBa_K2056005
BBa_K2056005 Version 1 (Component)
NOx(Oxides of Nitrogen) sensor device (pYeaR-Yellow chromoprotein)

P-alsT
BBa_K818300 Version 1 (Component)
Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.

BBa_K1351025
BBa_K1351025 Version 1 (Component)
P_<i>comAB</i>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>

BBa_K1351024
BBa_K1351024 Version 1 (Component)
P_<i>comC</i>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>

BBa_K730001
BBa_K730001 Version 1 (Component)
The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide.

BBa_K1758390
BBa_K1758390 Version 1 (Component)
His-tagged E. coli tryptophanase under control of the T7 promoter

BBa_K1758201
BBa_K1758201 Version 1 (Component)
His-tagged lac repressor under the control of the T7 promoter

BBa_K758003
BBa_K758003 Version 1 (Component)
UAS, this part consists of five UAS sequences and hsp70 TATA.

BBa_K2170110
BBa_K2170110 Version 1 (Component)
Intermediate of biotin binding receptor (CMV_BM40_BAP_NanoLuc_A3C5) in RFC[25] N-part

BBa_K382066
BBa_K382066 Version 1 (Component)
intron hairpin RNAi knockdown of Ger allergen in Basta plant vector

UAS-LacZ
BBa_K758005 Version 1 (Component)
UAS LacZ, this part expresses LacZ in the presence of GAL4