BBa_K918000
BBa_K918000 Version 1 (Component)
Mating Factor Alpha Secretion Tag

BBa_K1401009
BBa_K1401009 Version 1 (Component)
Transcriptional Unit with pTet-pBad tandem promoter

BBa_K1401010
BBa_K1401010 Version 1 (Component)
Transcriptional Unit with pBad-pTet tandem promoter

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

AraC O2
BBa_R0081 Version 1 (Component)
Inhibitor (AraC loop attachment with O2 site)

pSB3K3
pSB3K3 Version 1 (Component)
Low to medium copy BioBrick standard vector

BBa_K310028
BBa_K310028 Version 1 (Component)
Ptet + GadY

BBa_K2120001
BBa_K2120001 Version 1 (Component)
mutated ptet

BBa_K577885
BBa_K577885 Version 1 (Component)
pTet+GFPc

BBa_K123006
BBa_K123006 Version 1 (Component)
Ptet-BisdB

BBa_K1343017
BBa_K1343017 Version 1 (Component)
Ptet->tetR

Ptet-ChiXm
BBa_K564020 Version 1 (Component)
Mutated regulatory device based on sRNA.

BBa_K1875001
BBa_K1875001 Version 1 (Component)
This part contains a Kozak sequence

BBa_K1875003
BBa_K1875003 Version 1 (Component)
This part contains a GFP reporter

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

BBa_K2145007
BBa_K2145007 Version 1 (Component)
GFP/RFP reporter with spacer part (532bp)

BBa_K2145006
BBa_K2145006 Version 1 (Component)
GFP/RFP reporter with spacer part (532bp)

BBa_K2145004
BBa_K2145004 Version 1 (Component)
GFP/RFP reporter with spacer part (532bp)

BBa_K2145005
BBa_K2145005 Version 1 (Component)
GFP/RFP reporter with spacer part (532bp)

Dsb-Art175
BBa_K1659002 Version 1 (Component)
Artilysin Art-175 fused at N-terminal with DsbA signal peptide

BBa_K1416002
BBa_K1416002 Version 1 (Component)
pStG: a ncAA Synthetase/tRNA Pair Plasmid Standard

BBa_K563030
BBa_K563030 Version 1 (Component)
pEASY with first part of Tor2

BBa_K563033
BBa_K563033 Version 1 (Component)
pEASY with third part of Tor2

BBa_K563032
BBa_K563032 Version 1 (Component)
pEASY with second part of Tor2

Paro
BBa_K1884000 Version 1 (Component)
Paromomycin

BBa_I751001
BBa_I751001 Version 1 (Component)
pBR Biobrick-Tokyo Standard

BBa_K228003
BBa_K228003 Version 1 (Component)
Bistable part

Prefix-R
BBa_G1003 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

Suffix-F
BBa_G1002 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

BBa_J70394
BBa_J70394 Version 1 (Component)
J04450 {0,5:5,0} part

BBa_K2120202
BBa_K2120202 Version 1 (Component)
mutated ptet-3

BBa_K2120200
BBa_K2120200 Version 1 (Component)
mutated ptet-1

BBa_K2120203
BBa_K2120203 Version 1 (Component)
mutated ptet-25

BBa_K2120201
BBa_K2120201 Version 1 (Component)
mutated ptet-2

BBa_I742138
BBa_I742138 Version 1 (Component)
B-carotene (organge pigment) producing operon without PstI sites

BBa_K576003
BBa_K576003 Version 1 (Component)
Left Part of Self-Excising Ribozyme

BBa_K576004
BBa_K576004 Version 1 (Component)
Right Part of Self-Excising Ribozyme

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K542007
BBa_K542007 Version 1 (Component)
Unregulated TetR with pTet Regulated Arg-tagged ECFP

BBa_J33204
BBa_J33204 Version 1 (Component)
xylE reporter gene with rbs

BBa_J64019
BBa_J64019 Version 1 (Component)
invI SPI-1 Protein

BBa_I716377
BBa_I716377 Version 1 (Component)
Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD with cytochromes

BBa_I716379
BBa_I716379 Version 1 (Component)
Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD with AHSP

BBa_I716380
BBa_I716380 Version 1 (Component)
Biobricked Unmodified Hemoglobin Cassette in petDUET format with hemABCD with antioxidants

BBa_I716381
BBa_I716381 Version 1 (Component)
Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD with antioxidants

BBa_I716378
BBa_I716378 Version 1 (Component)
Biobricked Unmodified Hemoglobin Cassette in petDUET format with hemABCD with AHSP

BBa_I737001
BBa_I737001 Version 1 (Component)
protein generator for XXXX for making YYYY

BBa_K2066037
BBa_K2066037 Version 1 (Component)
pACT-Tet on UNS Standard

BBa_K209408
BBa_K209408 Version 1 (Component)
AarI C-D part, Intersectin (ITSN)

BBa_I756017
BBa_I756017 Version 1 (Component)
U6 promoter with tet operators