BBa_K584028BBa_K584028 Version 1 (Component)pLac-Lux hybrid promoter + INP(Ice Nucleating Protein Generator)
BBa_K649303BBa_K649303 Version 1 (Component)PlacIQ-RBS-ispS
BFPBBa_K592100 Version 1 (Component)Blue Fluorescent Protein (mTagBFP)
CspB | RFPBBa_K525234 Version 1 (Component)Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS
BBa_K539713BBa_K539713 Version 1 (Component)AlsS (alpha-acetolactate synthase protein)
BBa_K541915BBa_K541915 Version 1 (Component)Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide
BBa_K541715BBa_K541715 Version 1 (Component)Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide
BBa_K541925BBa_K541925 Version 1 (Component)Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and LipA signal peptide
BBa_K611018BBa_K611018 Version 1 (Component)Mutant Regulatory protein/promoter characterization plasmid
YFPBBa_K592101 Version 1 (Component)Yellow Fluorescent Protein (YFP)
BBa_K615001BBa_K615001 Version 1 (Component)Zinc finger-omega subunit fusion protein
BBa_K597100BBa_K597100 Version 1 (Component)avi-RFP [AviTagged red fluorescent protein]
CFPBBa_K566004 Version 1 (Component)CFP protein generator without transcription terminator
BBa_K597101BBa_K597101 Version 1 (Component)GFP-AviTag [AviTagged green fluorescent protein]
BBa_K823051BBa_K823051 Version 1 (Component)mKate2, a red monomeric fluorescent protein + RBS + Term
BBa_K748000BBa_K748000 Version 1 (Component)AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.
BBa_K748001BBa_K748001 Version 1 (Component)AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.
BBa_K784015BBa_K784015 Version 1 (Component)Phage Lambda fragment 1 - head proteins
BBa_K784016BBa_K784016 Version 1 (Component)Phage Lambda fragment 2 - tail proteins
BBa_K784017BBa_K784017 Version 1 (Component)Phage Lambda fragment 3 - tail tip proteins
BBa_K784018BBa_K784018 Version 1 (Component)Phage Lambda fragment 4 - tail fiber proteins
BBa_K932001BBa_K932001 Version 1 (Component)EnvZ Protein
BBa_K784022BBa_K784022 Version 1 (Component)Phage Lambda fragment 8 - lytic proteins
BBa_K758000BBa_K758000 Version 1 (Component)UAS , this is the enhancer . And this enhancer is used to identify the protein localization .
BBa_K758002BBa_K758002 Version 1 (Component)GAL4, this protein binds the UAS
BBa_K763005BBa_K763005 Version 1 (Component)pADH2 + Gene encoding YAP1 protein
BBa_K741020BBa_K741020 Version 1 (Component)plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T
BBa_K741021BBa_K741021 Version 1 (Component)plac-RBS-cI-T-pRM-anticro-T
BBa_K758004BBa_K758004 Version 1 (Component)GAL4, this part coding protein binds and activates the UAS sequence
UAS-EGFPBBa_K758006 Version 1 (Component)UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.
BBa_K812012BBa_K812012 Version 1 (Component)OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
BBa_K737016BBa_K737016 Version 1 (Component)This part conteins gvpA protein's coding sequence.
BBa_K737017BBa_K737017 Version 1 (Component)This part conteins gvpC-20psi protein
BBa_K737011BBa_K737011 Version 1 (Component)gvpC-GFP fusion protein with medium constitutive promoter
BBa_K737012BBa_K737012 Version 1 (Component)gvpC-GFP fusion protein UH with medium constitutive promoter
BBa_K737013BBa_K737013 Version 1 (Component)gvpC-GFP fusion protein
BBa_K737014BBa_K737014 Version 1 (Component)gvpC-GFP fusion protein UH
CBDclosBBa_K863111 Version 1 (Component)Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
BBa_K873000BBa_K873000 Version 1 (Component)heat shock 10kDa protein(hsp 10) groES coding resquence
BBa_K739004BBa_K739004 Version 1 (Component)Ax21 protein - signaling part
BBa_K883160BBa_K883160 Version 1 (Component)CCMV_Negative coat protein
BBa_K914002BBa_K914002 Version 1 (Component)repressilator RBS Colicin E2 Immunity protein
BBa_K883151BBa_K883151 Version 1 (Component)CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor
pLac ColE2BBa_K914001 Version 1 (Component)pLac Colicin E2 Immunity protein
gpSBBa_K836006 Version 1 (Component)Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K157010BBa_K157010 Version 1 (Component)glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac