BBa_K1202113
BBa_K1202113 Version 1 (Component)
TAT-RFP

BBa_K1347001
BBa_K1347001 Version 1 (Component)
RFP - Jaz1

BBa_K535008
BBa_K535008 Version 1 (Component)
repC - ctRNA

BBa_K1110000
BBa_K1110000 Version 1 (Component)
pGAPzalphaA+RFP

BBa_K1144016
BBa_K1144016 Version 1 (Component)
hoxN,RFP

BBa_K1071014
BBa_K1071014 Version 1 (Component)
RFP-MTS

BBa_K1933200
BBa_K1933200 Version 1 (Component)
constitutive expression of anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag

BBa_I732071
BBa_I732071 Version 1 (Component)
Double Reporters (LacZ and GFP-AAV)

BBa_K1109000
BBa_K1109000 Version 1 (Component)
FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence with r

BBa_K404236
BBa_K404236 Version 1 (Component)
[AAV2]Rep-VP123_p5TATAless (ViralBrick 453 RGD-587 HSPG-KO)

BBa_K404235
BBa_K404235 Version 1 (Component)
[AAV2]Rep-VP123_p5TATAless (ViralBrick 453 Z34C-587 HSPG-KO)

Nsense
BBa_M36356 Version 1 (Component)
A sensor that activates in the presence of nitrate in anaerobic conditions in E. coli.

BBa_K1185002
BBa_K1185002 Version 1 (Component)
HBsu-RFP

BBa_K861161
BBa_K861161 Version 1 (Component)
CRP with a RBS and a terminator

BBa_K1952010
BBa_K1952010 Version 1 (Component)
Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter

BBa_K602013
BBa_K602013 Version 1 (Component)
<i>E. coli</i> RecA promoter with CrtEBI reporter

BBa_K1952011
BBa_K1952011 Version 1 (Component)
Hydrazine Synthase subunit beta (Kust-2860) with LacZ reporter

BBa_K1797004
BBa_K1797004 Version 1 (Component)
Blue Light Sensing System With GFP And BFP Reporter

BBa_K1933201
BBa_K1933201 Version 1 (Component)
constitutive expression of anti-Norovirus GII.4 scFv fused to BclA with 6xHis tag

BBa_I745006
BBa_I745006 Version 1 (Component)
TRE-Bax-IRES2-EGFP

BBa_K2036013
BBa_K2036013 Version 1 (Component)
RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag

BBa_K1159314
BBa_K1159314 Version 1 (Component)
TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part

BBa_I715065
BBa_I715065 Version 1 (Component)
Burnt Pancake Tester with RE and backwards Tet resistance

dTomato
BBa_K1688019 Version 1 (Component)
dTomato, red fluorescent protein

BBa_S03711
BBa_S03711 Version 1 (Component)
RBS-Kan-RBS-RFP

BBa_K1918100
BBa_K1918100 Version 1 (Component)
TRE-Kozak-EBFP2N(1-154)

BBa_K199078
BBa_K199078 Version 1 (Component)
RBS RFP with CCAAU Addition

pSBBs3C
BBa_K823025 Version 1 (Component)
pSB_Bs3C-<i>luxABCDE</i> (lux reporter vector for B.subtilis)

BBa_K1845000
BBa_K1845000 Version 1 (Component)
RFP Reporter/Screening plasmid intermediate

BBa_K1078001
BBa_K1078001 Version 1 (Component)
Strong reporter device optimized for Pichia pastoris, activated by methanol

BBa_K1114302
BBa_K1114302 Version 1 (Component)
This is <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_B001

Propane1
BBa_K1655000 Version 1 (Component)
Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.

BBa_M11411
BBa_M11411 Version 1 (Component)
Type 2 promoter of lrtA gene. Gene is expressed during darkness. Potentially darkness-induced promot

BBa_I732093
BBa_I732093 Version 1 (Component)
Double Repoters (LacZ-alpha and GFP)

BBa_J24672
BBa_J24672 Version 1 (Component)
Tri-Stable Toggle with Flourescent Reporters

PhyB-908-(
BBa_K365011 Version 1 (Component)
Phytochrome B (aa 1-908) linked to a trimer of ClpX (aa 61-425)

PB642(L-C)
BBa_K365010 Version 1 (Component)
Phytochrome B (aa 1-642) linked to a trimer of ClpX (aa 61-425)

BBa_J120027
BBa_J120027 Version 1 (Component)
It is a composite part containing CH1 domain of human antibody along with GFP.

BBa_K079024
BBa_K079024 Version 1 (Component)
LacI repressor under the control of the J23118 costitutive promoter and Lac 1 operator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K544021
BBa_K544021 Version 1 (Component)
dKeima-Red

BBa_J107023
BBa_J107023 Version 1 (Component)
R0061 measurement system (with RFP)

BBa_K1061002
BBa_K1061002 Version 1 (Component)
RIP 1

BBa_J100204
BBa_J100204 Version 1 (Component)
actClone Red

BBa_J100205
BBa_J100205 Version 1 (Component)
repClone Red

BBa_J119083
BBa_J119083 Version 1 (Component)
RFP Node

BBa_K1391017
BBa_K1391017 Version 1 (Component)
MAV1212-RFP

BBa_K1728018
BBa_K1728018 Version 1 (Component)
DUSP1 toehold switch RNA sensor with T7 promoter & luciferase reporter

BBa_K1728016
BBa_K1728016 Version 1 (Component)
IL8 toehold switch RNA sensor with T7 promoter & luciferase reporter

BBa_K1728019
BBa_K1728019 Version 1 (Component)
SAT toehold switch RNA sensor with T7 promoter & luciferase reporter