BBa_K798014
BBa_K798014 Version 1 (Component)
Recognition sequence of ribozyme sequence

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K351007
BBa_K351007 Version 1 (Component)
first Ig-like region of Human basic FGFR with signal peptide for s.pombe surface

BBa_K2116028
BBa_K2116028 Version 1 (Component)
bxb1 integrase under the control of nitric oxide and AHL responsive AND gate

LigA
BBa_K525710 Version 1 (Component)
DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag

PcotYZ
BBa_K823030 Version 1 (Component)
P_<i>cotYZ</i>: <i>B. subtilis</i> promoter regulating expression of spore crust proteins

BBa_K1456012
BBa_K1456012 Version 1 (Component)
Human Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His

BBa_K1456010
BBa_K1456010 Version 1 (Component)
Glutathione Peroxidase (GPx) enzyme reverse primer with restriction site, two stop codon and 6X His

BBa_K1456008
BBa_K1456008 Version 1 (Component)
Superoxide Dismutase enzyme reverse primer with restriction site, two stop codon and 6X His-Tag

BBa_M45091
BBa_M45091 Version 1 (Component)
AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa

BBa_K525161
BBa_K525161 Version 1 (Component)
Fusion Protein of Luciferase and cspB from Corynebacterium glutamicum with TAT-Sequence and lipid an

BBa_K2055014
BBa_K2055014 Version 1 (Component)
Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron.

BBa_K1753000
BBa_K1753000 Version 1 (Component)
the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)

BBa_M31539
BBa_M31539 Version 1 (Component)
These are the genes in M13K07 responsible for forming the 'tip' of the phage.

BBa_K2151008
BBa_K2151008 Version 1 (Component)
p32-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site

BBa_K2151007
BBa_K2151007 Version 1 (Component)
p25-I13500: GFP under control of medium constitutive promoter and strong ribosome binding site

BBa_K2151006
BBa_K2151006 Version 1 (Component)
pHLBA-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site

BBa_K2082008
BBa_K2082008 Version 1 (Component)
Zika E-Protein: Fusion of Zika E-Protein with cI (434) regulatory protein with cMyc linker

BBa_K1758353
BBa_K1758353 Version 1 (Component)
Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promote

BBa_K1051801
BBa_K1051801 Version 1 (Component)
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

BBa_K1688007
BBa_K1688007 Version 1 (Component)
ModLac laccase with His-tag and HlyA export tag (inc RBS and J23110 promoter)

BBa_J119316
BBa_J119316 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A & B and the RBS in the junction B & C)

BBa_K1933200
BBa_K1933200 Version 1 (Component)
constitutive expression of anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag

BBa_K1109000
BBa_K1109000 Version 1 (Component)
FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence with r

BBa_K1638010
BBa_K1638010 Version 1 (Component)
T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> with GFP reporter

BBa_K1933201
BBa_K1933201 Version 1 (Component)
constitutive expression of anti-Norovirus GII.4 scFv fused to BclA with 6xHis tag

BBa_K1638009
BBa_K1638009 Version 1 (Component)
T18 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> with GFP reporter

BBa_K1189020
BBa_K1189020 Version 1 (Component)
Light chain human ferritin with his-tag and E-coil under the lacI inducible promoter

BBa_K1189019
BBa_K1189019 Version 1 (Component)
Heavy chain human ferritin with his-tag and E-coil under the lacI inducible promoter

BBa_M1532
BBa_M1532 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1533
BBa_M1533 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

BBa_K1159314
BBa_K1159314 Version 1 (Component)
TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part

BBa_K1162307
BBa_K1162307 Version 1 (Component)
WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag

Propane1
BBa_K1655000 Version 1 (Component)
Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.

BBa_K1088002
BBa_K1088002 Version 1 (Component)
Reporter system to quantify protein expression of B. sub 1-deoxyxylulose-5-phosphate synthase (Dxs)

BBa_K2123116
BBa_K2123116 Version 1 (Component)
Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)

BBa_K079026
BBa_K079026 Version 1 (Component)
GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter

BBa_K079024
BBa_K079024 Version 1 (Component)
LacI repressor under the control of the J23118 costitutive promoter and Lac 1 operator

iGEM 2019 Plates
iGEM_2019_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2019

iGEM 2018 Plates
iGEM_2018_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2018

BBa_J36849
BBa_J36849 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag

BBa_J36851
BBa_J36851 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag

BBa_M11051
BBa_M11051 Version 1 (Component)
Captures light energy and uses it to move protons across the membrane out of the cell.

BBa_K1172304
BBa_K1172304 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K1638030
BBa_K1638030 Version 1 (Component)
Leucine zipper fused to T25 domain of <i>cyaA</i> with cAMP-induced RFP generator

BBa_K1638031
BBa_K1638031 Version 1 (Component)
Leucine zipper fused to T18 domain of <i>cyaA</i> with cAMP-induced RFP generator

BBa_K2047001
BBa_K2047001 Version 1 (Component)
Fluorescent reporter system with stem-loop, GFP_Stem-loop with free energy of -25.6 kcal/mol

BBa_K2082231
BBa_K2082231 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind