OriTRP4BBa_K1439000 Version 1 (Component)Origin of transfer for the RP4-plasmid nic region.
dddDBBa_K1312002 Version 1 (Component)dddD gene from Ruegeria pomeroyi.
BBa_K1413041BBa_K1413041 Version 1 (Component)Mutation of OriVR6Kgamma origin of replication (ori)
BBa_K1413042BBa_K1413042 Version 1 (Component)OriVR6Kgamma origin of replication (ori) 2
BBa_K2010007BBa_K2010007 Version 1 (Component)pelB + sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
BBa_K2055014BBa_K2055014 Version 1 (Component)Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron.
BBa_K2055015BBa_K2055015 Version 1 (Component)Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe
scFvBBa_K1933002 Version 1 (Component)anti-Norovirus GII.4 scFv
BBa_K1933200BBa_K1933200 Version 1 (Component)constitutive expression of anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag
BBa_K1933201BBa_K1933201 Version 1 (Component)constitutive expression of anti-Norovirus GII.4 scFv fused to BclA with 6xHis tag
BBa_K2022012BBa_K2022012 Version 1 (Component)INPNC-GFP, i.e. membrane-anchored GFP
BBa_K1947021BBa_K1947021 Version 1 (Component)This part serves as a catch system expressed in E. coli.
BBa_K1947023BBa_K1947023 Version 1 (Component)This part serves as a catch system expressed in <i>E. coli.
BBa_K2022013BBa_K2022013 Version 1 (Component)INPNC-RFP, i.e. membrane-anchored RFP
BBa_K2179000BBa_K2179000 Version 1 (Component)chlorite dismutase CDS+RBS from I. deschloratans
Cld(-SP)BBa_K2179003 Version 1 (Component)Chlorite Dismutase CDS+RBS from I. dechloratans with His-tag
BBa_J119405BBa_J119405 Version 1 (Component)(pSB1A2-BR) Deletion of the original -35 sequence
OriBBa_K320000 Version 1 (Component)Asaia replication origin
ori+TetRBBa_K320003 Version 1 (Component)Origin for Asaia and resistance to Tetracycline
Ori+KanBBa_K320004 Version 1 (Component)Origin for Asaia and resistance to Kanamycin
ori+AmpBBa_K320011 Version 1 (Component)Origin for Asaia and resistance to Ampicillin
BBa_K322710BBa_K322710 Version 1 (Component)Marker construct which can be used to target tnaA in E.coli.
BBa_K524000BBa_K524000 Version 1 (Component)Heat sensitive origin of replication (oriR101 & repA101-ts)
BBa_K607035BBa_K607035 Version 1 (Component)pUC18 replication origin
BBa_K625004BBa_K625004 Version 1 (Component)Minimal pBR322 pMB1 origin
BBa_K640002BBa_K640002 Version 1 (Component)oriT - Interspecies origin of transfer
BBa_K640003BBa_K640003 Version 1 (Component)Ori1600- Pseudomonas origin of replication
BBa_J72158BBa_J72158 Version 1 (Component)minimal ColE2 origin of replication
BBa_J72161BBa_J72161 Version 1 (Component)ColE2 origin of replication
BBa_J72215BBa_J72215 Version 1 (Component)MC1061 O16::rbsI.repA+FRT
BBa_J72208BBa_J72208 Version 1 (Component)Kanamycin resistant BAC with R6K origin
BBa_J72210BBa_J72210 Version 1 (Component)Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site
BBa_K759001BBa_K759001 Version 1 (Component)Aggregation Module inducible by arabinose in E.coli.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
OriTRBBa_J01003 Version 1 (Component)OriT-R (Origin of transfer for the R-plasmid nic region)
BBa_K116000BBa_K116000 Version 1 (Component)A homolog of OCT3 (organic cation transporter 3) from E coli. K12
BBa_K112125BBa_K112125 Version 1 (Component)OriV origin of replication
BBa_K112139BBa_K112139 Version 1 (Component)oriR6K conditional replication origin
BBa_K112143BBa_K112143 Version 1 (Component)Entry vector with OriV replication origin
BBa_J70027BBa_J70027 Version 1 (Component)Mesoplasma florum plasmid origin
BBa_J70342BBa_J70342 Version 1 (Component)J70315psri.f.1: J70315 psri forward part for pcr of S03621 (single stranded)
BBa_J70343BBa_J70343 Version 1 (Component)J70315psri.r.1: J70315 psri reverse part for pcr of S03621 (single stranded)
BBa_J70310BBa_J70310 Version 1 (Component)Mesoplasma florum pBG7AU plasmid origin
BBa_J70500BBa_J70500 Version 1 (Component)pWV01 broad host range plasmid origin and rep protein
BBa_K187424BBa_K187424 Version 1 (Component)Origin in AB, Forward primer
BBa_K187425BBa_K187425 Version 1 (Component)Origin in AB, Reverse primer
BBa_K187426BBa_K187426 Version 1 (Component)Origin in BA, Forward primer
BBa_K187427BBa_K187427 Version 1 (Component)Origin in BA, Reverse primer
BBa_K299107BBa_K299107 Version 1 (Component)T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)