ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K1172901
BBa_K1172901 Version 1 (Component)
Alanine racemase from ''E. coli''

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K733001
BBa_K733001 Version 1 (Component)
<i>Ptms</i> - a constitutive promoter in <i>E. coli</i> and <i>B. subtilis</i>.

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K325210
BBa_K325210 Version 1 (Component)
Red Firefly Luciferase and LRE<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

phlF
BBa_K1725040 Version 1 (Component)
<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5

p_qrr4
BBa_K131017 Version 1 (Component)
p_qrr4 from <I>Vibrio harveyi</I>

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_C0011
BBa_C0011 Version 1 (Component)
ccdB gene from <I> E. coli </I>

BBa_K325100
BBa_K325100 Version 1 (Component)
EPIC Firefly Luciferase and LRE <BR><i>P. Pyralis<BR>(E. coli optimised)</i>

BBa_K325903
BBa_K325903 Version 1 (Component)
luxEG <BR><i>V. Fischeri<BR>(E. coli optimised)</i>

BBa_K733010
BBa_K733010 Version 1 (Component)
<i>Ptms</i>+RBS+<i>ydcD</i>.

BBa_K531006
BBa_K531006 Version 1 (Component)
Optimized esp gene for <i>C. crescentus</i>

BBa_K733013
BBa_K733013 Version 1 (Component)
<i>Pveg</i>+R.B.S.

BBa_K733006
BBa_K733006 Version 1 (Component)
<i>ydjM</i>+<i>Bmp2</i>

BBa_K1638003
BBa_K1638003 Version 1 (Component)
T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> (IPTG inducible)

BBa_K1315001
BBa_K1315001 Version 1 (Component)
The <i>pqsA</i> promoter from <i>Pseudomonas</i>

PTA
BBa_K901016 Version 1 (Component)
Phosphotransacetylase (PTA) from <i>Escherichia coli</i>

BBa_K1315008
BBa_K1315008 Version 1 (Component)
The <i>cblD</i> promoter from <i>Burkholderia</i>

BBa_K733012
BBa_K733012 Version 1 (Component)
<i>xylR</i>+<i>PxylA</i>+RBS+<i>ydcE</i>+<i>Ptms</i>+RBS+<i>ydcD</i>: Cell Growth Inhibition Device

BBa_K1315005
BBa_K1315005 Version 1 (Component)
The <i>clp</i> gene from <i>Xanthomonas campestris</i>

BBa_K1315003
BBa_K1315003 Version 1 (Component)
The <i>rpfG</i> gene from <i>Xanthomonas campestris</i>

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

BBa_K1315006
BBa_K1315006 Version 1 (Component)
The <i>manA</i> promoter from <i>Xanthomonas campestris</i>

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)