URA5
GenBank_CM000046_Region_302227_303005 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

URA5
GenBank_CM000046_Region_301512_302226 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

URA5
GenBank_CM000046_Region_303006_303540 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

cI 434
BBa_R0052 Version 1 (Component)
Promoter (434 cI regulated)

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

Prm +
BBa_I12006 Version 1 (Component)
Modified lamdba Prm promoter (repressed by 434 cI)

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_I12054
BBa_I12054 Version 1 (Component)
B protein (434 cI) subassembly for modified BL oscillator

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>

cI HK022
BBa_C0050 Version 1 (Component)
cI repressor from phage HK022 (+LVA?)

BBa_C0056
BBa_C0056 Version 1 (Component)
cI repressor from phage 434 (no LVA)

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

LuxR
BBa_I0462 Version 1 (Component)
luxR Protein Generator

tetA(C)b
BBa_J31006 Version 1 (Component)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

BBa_P0152
BBa_P0152 Version 1 (Component)
PoPS -> cI (434) [S0166]

BBa_I12056
BBa_I12056 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12036

BBa_I12055
BBa_I12055 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12006

BBa_I12057
BBa_I12057 Version 1 (Component)
A protein (lambda cI) subassembly for modified BL oscillator based on I12040

BBa_I12036
BBa_I12036 Version 1 (Component)
Modified lamdba Prm promoter (cooperative repression by 434 cI)

BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

Pveg-spoVG
BBa_K143053 Version 1 (Component)
Promoter Pveg and RBS spoVG for B. subtilis

BBa_K209402
BBa_K209402 Version 1 (Component)
AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

cI 434
BBa_R1052 Version 1 (Component)
Promoter, Standard (434 cI regulated)<br>

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_P0451
BBa_P0451 Version 1 (Component)
PoPS -> cI (lambda) [S0153]

Pveg
BBa_K143012 Version 1 (Component)
Promoter veg a constitutive promoter for B. subtilis

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

cheZ
BBa_K629003 Version 1 (Component)
cheZ, chemotaxis regulator, protein phosphatase for CheY

cI
BBa_I9200 Version 1 (Component)
cI lambda

cI HK022
BBa_R0050 Version 1 (Component)
Promoter (HK022 cI regulated)

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_I12043
BBa_I12043 Version 1 (Component)
Activator for BL oscillator with reporter protein