Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_B0070
BBa_B0070 Version 1 (Component)
Specialized RBS modified from that of B0036 (Brink et al.)

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

BBa_K2066506
BBa_K2066506 Version 1 (Component)
RiboJ (Ribozyme Insulator) Lou et. al. 2012

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

BBa_J56012
BBa_J56012 Version 1 (Component)
Invertible sequence of dna includes Ptrc promoter

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_B0073
BBa_B0073 Version 1 (Component)
Specialized RBS

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

BBa_B0016
BBa_B0016 Version 1 (Component)
Terminator (T7 RNAP specific, T_Phi)

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_E0422
BBa_E0422 Version 1 (Component)
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)

BBa_K2066508
BBa_K2066508 Version 1 (Component)
Modified pLacO-1 Promoter (Lou et. al 2012)

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

BBa_E0420
BBa_E0420 Version 1 (Component)
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)

pSC101
BBa_I50041 Version 1 (Component)
pSC101 origin of replication, reverse

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_K2066510
BBa_K2066510 Version 1 (Component)
Strong RBS from Lou et. al 2012

BBa_B0035
BBa_B0035 Version 1 (Component)
RBS (B0030 derivative)

Prm +
BBa_I12006 Version 1 (Component)
Modified lamdba Prm promoter (repressed by 434 cI)

lacI
BBa_Q04121 Version 1 (Component)
QPI (B0034.C0012.B0015.R0011)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_K2066021
BBa_K2066021 Version 1 (Component)
UNS 5 Sequence, from Torella et al., 2013

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

tetR
BBa_Q04400 Version 1 (Component)
QPI (B0034.C0040.B0015.R0040)