Cas9
Cas9 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

mRFPr
BBa_J31008 Version 1 (Component)
RFP reverse

mRFP1
BBa_I13502 Version 1 (Component)
Screening plasmid intermediate

BBa_K2019001
BBa_K2019001 Version 1 (Component)
mRFP+gRNA Template (for saCas9)

mCherry
BBa_J06505 Version 1 (Component)
monomeric RFP optimized for bacteria

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

mRFP
BBa_K1615089 Version 1 (Component)
mRFP RFC25

mRFP1
BBa_E2050 Version 1 (Component)
derivative of mRFP1, yeast-optimized

BBa_K228001
BBa_K228001 Version 1 (Component)
SupD-tRNA

BBa_K081014
BBa_K081014 Version 1 (Component)
RFP protein generator - PoPS->RFP

BBa_K1699002
BBa_K1699002 Version 1 (Component)
gRNA for SaCas9 targeting human ubiquitin B gene

BBa_I13521
BBa_I13521 Version 1 (Component)
Ptet mRFP

mRFP sel.
BBa_K1362422 Version 1 (Component)
mRFP selection marker with outward BsaI restriction sites

BBa_K115002
BBa_K115002 Version 1 (Component)
RNA thermometer (FourU)

BBa_K1699004
BBa_K1699004 Version 1 (Component)
gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter

BBa_K115001
BBa_K115001 Version 1 (Component)
RNA thermometer (ROSE)

BBa_I2032
BBa_I2032 Version 1 (Component)
T7 RNA polymerase

mRFP1
BBa_J04650 Version 1 (Component)
E1010.B0015

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

BBa_K1699003
BBa_K1699003 Version 1 (Component)
gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm

BBa_J23008
BBa_J23008 Version 1 (Component)
[key3c] riboregulator for lock3 variants

BBa_K1026003
BBa_K1026003 Version 1 (Component)
gRNA targeting mRFP

BBa_I13401
BBa_I13401 Version 1 (Component)
GFP reporter for RHS of library test constructs

BBa_K2019002
BBa_K2019002 Version 1 (Component)
mRFP + sgRNA targeting mRFP Coding Region (saCas9)

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

BBa_K1150000
BBa_K1150000 Version 1 (Component)
dCas9

lox
BBa_J61046 Version 1 (Component)
[Lox] site for recombination

BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

BBa_K199118
BBa_K199118 Version 1 (Component)
T7 promoter + RBS + RFP

RFPi
BBa_K1062000 Version 1 (Component)
Guide RNA (gRNA) target for RFP