GFP_FreibuBBa_K863120 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)
BBa_K782057BBa_K782057 Version 1 (Component)SacI/SpeI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors
BBa_K782058BBa_K782058 Version 1 (Component)SacI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors
CBDclos(T7BBa_K863112 Version 1 (Component)Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)
comDEBBa_K1351016 Version 1 (Component)comDE Quorum Sensing two component system (Freiburg Standard)
RFC105 CCBBa_K1362419 Version 1 (Component)C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC
BBa_K1321339BBa_K1321339 Version 1 (Component)CBDcenA+Linker, RFC 25 standard
BBa_K2066037BBa_K2066037 Version 1 (Component)pACT-Tet on UNS Standard
BBa_K2066017BBa_K2066017 Version 1 (Component)240x TetO on UNS Standard
BBa_K2066016BBa_K2066016 Version 1 (Component)Constitutive Lac Repressor on UNS Standard
BBa_K2066038BBa_K2066038 Version 1 (Component)Sigma54 Enhancer 57S, no DT, UNS standard
BBa_K2066039BBa_K2066039 Version 1 (Component)Sigma54 Enhancer 57S with DT, UNS Standard
BBa_K2066112BBa_K2066112 Version 1 (Component)Synthetic Enhancer Project: Ntr promoter driven NRII2302 (mut) on UNS Standard
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
cI HK022BBa_R1050 Version 1 (Component)Promoter, Standard (HK022 cI regulated) <br>
BBa_K358001BBa_K358001 Version 1 (Component)Measurement Standard
BBa_J95045BBa_J95045 Version 1 (Component)reference standard for Promoter measurement (B0015) for E coli
BBa_J95049BBa_J95049 Version 1 (Component)Terminator measurement reference standard (B0015)
BBa_K648005BBa_K648005 Version 1 (Component)Short Fusion Protein Linker: GGSG with standard 25 prefix/suffix
BBa_K648006BBa_K648006 Version 1 (Component)Long 10AA Fusion Protein Linker with Standard 25 Prefix/Suffix
BBa_K648007BBa_K648007 Version 1 (Component)Medium 6AA Fusion Protein Linker: GGSGGS with Standard 25 Prefix/Suffix
BBa_K648008BBa_K648008 Version 1 (Component)TEV protease cleaveage site with Standard 25 Prefix/Suffix
BBa_K648009BBa_K648009 Version 1 (Component)RecA Cleavage Site with Standard 25 Prefix/Suffix
BBa_K648011BBa_K648011 Version 1 (Component)Standard 25-Ready Xyle Reporter
BBa_K648012BBa_K648012 Version 1 (Component)LacZa wtih Standard 25 Prefix/Suffix
BBa_K648013BBa_K648013 Version 1 (Component)GFP with Standard 25 Prefix/Suffix
BBa_K549021BBa_K549021 Version 1 (Component)pabA with Freiburg-standard in pSB1C3
BBa_K549025BBa_K549025 Version 1 (Component)petE2 in Freiburg-standard
K592200BBa_K592200 Version 1 (Component)Low to medium copy BioBrick standard vector
BBa_K823038BBa_K823038 Version 1 (Component)Strep-tag (Freiburg standard+RBS)
BBa_K823039BBa_K823039 Version 1 (Component)gfpmut1 (B. subtilis optimized + Freiburg standard)
CBDcexBBa_K863101 Version 1 (Component)Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)
CBDclosBBa_K863111 Version 1 (Component)Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
CBDcex(T7)BBa_K863102 Version 1 (Component)Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
linkerBBa_K157009 Version 1 (Component)Split fluorophore linker; Freiburg standard
HisBBa_K157011 Version 1 (Component)His affinity tag; Freiburg standard
BBa_K157012BBa_K157012 Version 1 (Component)Strep affinity tag II; Freiburg standard
linkerBBa_K157013 Version 1 (Component)15 aa flexible glycine-serine protein domain linker; Freiburg standard
BBa_J69591BBa_J69591 Version 1 (Component)GFP reporter device - using Standard Promoter (1RPU)
lux pRBBa_R1062 Version 1 (Component)Promoter, Standard (luxR and HSL regulated -- lux pR)<br>
BBa_I20285BBa_I20285 Version 1 (Component)Bxb1 Excisionase-Freiburg standard
BBa_I20286BBa_I20286 Version 1 (Component)TP901-1 integrase- Freiburg standard
BBa_I20287BBa_I20287 Version 1 (Component)TP901-1 Excisionase-Freiburg standard
BBa_I20288BBa_I20288 Version 1 (Component)PhiRVI integrase- Freiburg standard
BBa_I20289BBa_I20289 Version 1 (Component)PhiRVI excisionase-Freiburg Standard
Bxb1BBa_I20284 Version 1 (Component)Bacteriophage Bxb1 Integrase-Freiburg standard