BBa_K782018
BBa_K782018 Version 1 (Component)
10x[TALB] operator_CMV promoter_mCitrine

BBa_K782019
BBa_K782019 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_fLuciferase

BBa_K782020
BBa_K782020 Version 1 (Component)
10x[TALA] operator_CMV promoter_fLuciferase

BBa_K782021
BBa_K782021 Version 1 (Component)
10x[TALB] operator_CMV promoter_fLuciferase

BBa_K782022
BBa_K782022 Version 1 (Component)
12x[TALD] operator_CMV promoter_fLuciferase

BBa_K782023
BBa_K782023 Version 1 (Component)
12x[NicTAL] operator_CMV promoter_fLuciferase

BBa_K782024
BBa_K782024 Version 1 (Component)
2x[NicTAL]+2x[TALD] operator_minimal promoter_mCitrine

BBa_K782025
BBa_K782025 Version 1 (Component)
4x[NicTAL]+4x[TALD] operator_minimal promoter_mCitrine

BBa_K782026
BBa_K782026 Version 1 (Component)
7x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782027
BBa_K782027 Version 1 (Component)
7x[TALA] operator_minimal promoter_mCitrine

BBa_K782028
BBa_K782028 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_mCitrine

BBa_K782029
BBa_K782029 Version 1 (Component)
10x[TALA] operator_minimal promoter_mCitrine

BBa_K782030
BBa_K782030 Version 1 (Component)
10x[TALB] operator_minimal promoter_mCitrine

BBa_K782031
BBa_K782031 Version 1 (Component)
12x[TALD] operator_minimal promoter_mCitrine

BBa_K782032
BBa_K782032 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_fLuciferase

BBa_K782033
BBa_K782033 Version 1 (Component)
10x[TALA] operator_minimal promoter_fLuciferase

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_S04136
BBa_S04136 Version 1 (Component)
terminator and cI promoter

BBa_K098995
BBa_K098995 Version 1 (Component)
heat sensitive cI QPI with high promoter

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098986
BBa_K098986 Version 1 (Component)
lac QPI driven by high promoter

BBa_K098985
BBa_K098985 Version 1 (Component)
lac QPI driven by low promoter

BBa_K113010
BBa_K113010 Version 1 (Component)
overlapping T7 promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K113011
BBa_K113011 Version 1 (Component)
more overlapping T7 promoter

BBa_K105026
BBa_K105026 Version 1 (Component)
Gal1 promoter

BBa_K098981
BBa_K098981 Version 1 (Component)
tet QPI driven by high promoter

BBa_K098980
BBa_K098980 Version 1 (Component)
tet QPI driven by low promoter

BBa_K113012
BBa_K113012 Version 1 (Component)
weaken overlapping T7 promoter

BBa_K113014
BBa_K113014 Version 1 (Component)
truncated T7 promoter->GFP

BBa_K113015
BBa_K113015 Version 1 (Component)
bidirectional intact T7 promoters->GFP/RFP

BBa_K105027
BBa_K105027 Version 1 (Component)
cyc100 minimal promoter

BBa_K113016
BBa_K113016 Version 1 (Component)
overlapping T7 promoter->GFP/RFP

BBa_K113017
BBa_K113017 Version 1 (Component)
more overlapping T7 promoter->GFP/RFP

BBa_K113018
BBa_K113018 Version 1 (Component)
weaken overlapping T7 promoter->GFP/RFP

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_K105028
BBa_K105028 Version 1 (Component)
cyc70 minimal promoter

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

BBa_K105029
BBa_K105029 Version 1 (Component)
cyc43 minimal promoter

BBa_K105030
BBa_K105030 Version 1 (Component)
cyc28 minimal promoter

BBa_K105031
BBa_K105031 Version 1 (Component)
cyc16 minimal promoter

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

cI 434
BBa_R0052 Version 1 (Component)
Promoter (434 cI regulated)

rTT+pR
BBa_K093003 Version 1 (Component)
Convergent Promoter System: Forward Module: rTT+lambda pR

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_K090501
BBa_K090501 Version 1 (Component)
Gram-Positive IPTG-Inducible Promoter

BBa_K090502
BBa_K090502 Version 1 (Component)
Gram-Positive Xylose-Inducible Promoter