GFPLVA
BBa_K411207 Version 1 (Component)
Green Fluorescent Protein with LVA tag (GFPLVA)

BBa_K311003
BBa_K311003 Version 1 (Component)
Bacterial Microcompartment Proteins

BBa_K311004
BBa_K311004 Version 1 (Component)
Complement of Ethanolamine Utilization Microcompartment proteins from Salmonella LT2

RFPLVA
BBa_K411208 Version 1 (Component)
Red Fluorescent Protein with LVA tag (RFPLVA)

BBa_K656007
BBa_K656007 Version 1 (Component)
Cyan fluorescent protein bound to dockerin of C. thermocellum

BBa_K523012
BBa_K523012 Version 1 (Component)
Enhanced Yellow Fluorescent Protein for BioSandwich

BBa_K608404
BBa_K608404 Version 1 (Component)
IPTG-inducible Promoter with plastic binding domain-tagged GFP

BBa_J52028
BBa_J52028 Version 1 (Component)
green fluorescent protein derived from A. victoria GFP (SwissProt: P42212) with PEST191 tag

BBa_K631013
BBa_K631013 Version 1 (Component)
RFP (Fluorescent protein; for use in C. elegans)

BBa_K638401
BBa_K638401 Version 1 (Component)
Arabinose inducible TorA Export Tagged Reflectin A1 Poly His generator

BFP
BBa_K592100 Version 1 (Component)
Blue Fluorescent Protein (mTagBFP)

YFP
BBa_K592101 Version 1 (Component)
Yellow Fluorescent Protein (YFP)

BBa_K642000
BBa_K642000 Version 1 (Component)
TetR repressor tagged with yBFP

BBa_K642004
BBa_K642004 Version 1 (Component)
TetR repressor tagged with yBFP and a VP16 activation domain

BBa_K642001
BBa_K642001 Version 1 (Component)
LacI repressor tagged with yBFP

BBa_K642002
BBa_K642002 Version 1 (Component)
cI repressor tagged with yBFP

BBa_K642003
BBa_K642003 Version 1 (Component)
LexA repressor tagged with yBFP

BBa_K642005
BBa_K642005 Version 1 (Component)
LacI repressor tagged with yBFP and a VP16 activation domain

BBa_K642006
BBa_K642006 Version 1 (Component)
cI repressor tagged with yBFP and a VP16 activation domain

BBa_K542005
BBa_K542005 Version 1 (Component)
pTet Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

BBa_K542006
BBa_K542006 Version 1 (Component)
pBAD Inverse-Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

BBa_K542007
BBa_K542007 Version 1 (Component)
Unregulated TetR with pTet Regulated Arg-tagged ECFP

BBa_K542008
BBa_K542008 Version 1 (Component)
pLacI Regulated Lumazine Synthase and pBAD Inverse-Regulated Arg-tagged ECFP and EYFP

BBa_K597100
BBa_K597100 Version 1 (Component)
avi-RFP [AviTagged red fluorescent protein]

BBa_K597101
BBa_K597101 Version 1 (Component)
GFP-AviTag [AviTagged green fluorescent protein]

BBa_K597102
BBa_K597102 Version 1 (Component)
VioA-AviTag [AviTagged violacein enzyme A]

BBa_K597103
BBa_K597103 Version 1 (Component)
VioB-AviTag [AviTagged violacein enzyme B]

BBa_K597104
BBa_K597104 Version 1 (Component)
VioE-AviTag [AviTagged violacein enzyme E]

BBa_K823051
BBa_K823051 Version 1 (Component)
mKate2, a red monomeric fluorescent protein + RBS + Term

BBa_K784015
BBa_K784015 Version 1 (Component)
Phage Lambda fragment 1 - head proteins

BBa_K784016
BBa_K784016 Version 1 (Component)
Phage Lambda fragment 2 - tail proteins

BBa_K784017
BBa_K784017 Version 1 (Component)
Phage Lambda fragment 3 - tail tip proteins

BBa_K784018
BBa_K784018 Version 1 (Component)
Phage Lambda fragment 4 - tail fiber proteins

BBa_K784022
BBa_K784022 Version 1 (Component)
Phage Lambda fragment 8 - lytic proteins

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

SBFP2
BBa_K156010 Version 1 (Component)
SBFP2 (strongly enhanced blue fluorescent protein)

lexA
BBa_K165020 Version 1 (Component)
LexA Synthetic Activator (untagged)

BBa_K165021
BBa_K165021 Version 1 (Component)
Kozak + LexA Synthetic Activator (untagged)

BBa_K165022
BBa_K165022 Version 1 (Component)
Zif268-HIV synthetic activator (untagged)

BBa_K165023
BBa_K165023 Version 1 (Component)
Kozak + Zif268-HIV synthetic activator (untagged)

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K165050
BBa_K165050 Version 1 (Component)
Full Gli1 Repressor (tagged with mCherryx2)

BBa_K165051
BBa_K165051 Version 1 (Component)
Full YY1 Repressor (tagged with mCherryx2)

BBa_K165052
BBa_K165052 Version 1 (Component)
Full Zif268-HIV Repressor (tagged with mCherryx2)

BBa_K165053
BBa_K165053 Version 1 (Component)
Full LexA Repressor (tagged with CFPx2)

BBa_K165054
BBa_K165054 Version 1 (Component)
Full Gli1 Repressor (tagged with CFPx2)

BBa_K165080
BBa_K165080 Version 1 (Component)
pGAL1 + Untagged LexA activator on pRS305