BBa_K1172305BBa_K1172305 Version 1 (Component)Riboflavin synthesis gene cluster from s. oneidensis under control of a medium Anderson promoter
BBa_M45091BBa_M45091 Version 1 (Component)AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa
BBa_K525161BBa_K525161 Version 1 (Component)Fusion Protein of Luciferase and cspB from Corynebacterium glutamicum with TAT-Sequence and lipid an
BBa_R4030BBa_R4030 Version 1 (Component)PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)
BBa_K1753000BBa_K1753000 Version 1 (Component)the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)
BBa_M31539BBa_M31539 Version 1 (Component)These are the genes in M13K07 responsible for forming the 'tip' of the phage.
BBa_K2151008BBa_K2151008 Version 1 (Component)p32-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site
BBa_K2151007BBa_K2151007 Version 1 (Component)p25-I13500: GFP under control of medium constitutive promoter and strong ribosome binding site
BBa_K2151006BBa_K2151006 Version 1 (Component)pHLBA-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site
BBa_K1051801BBa_K1051801 Version 1 (Component)Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib
BBa_K1109000BBa_K1109000 Version 1 (Component)FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence with r
NsenseBBa_M36356 Version 1 (Component)A sensor that activates in the presence of nitrate in anaerobic conditions in E. coli.
BBa_K165100BBa_K165100 Version 1 (Component)Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*
BBa_K1638010BBa_K1638010 Version 1 (Component)T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> with GFP reporter
BBa_K1638009BBa_K1638009 Version 1 (Component)T18 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> with GFP reporter
BBa_K1159314BBa_K1159314 Version 1 (Component)TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part
BBa_K145112BBa_K145112 Version 1 (Component)cI under T7 and PR<sub>R</sub> dual promotor
BBa_K1114302BBa_K1114302 Version 1 (Component)This is <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_B001
Propane1BBa_K1655000 Version 1 (Component)Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.
BBa_M11411BBa_M11411 Version 1 (Component)Type 2 promoter of lrtA gene. Gene is expressed during darkness. Potentially darkness-induced promot
PhyB-908-(BBa_K365011 Version 1 (Component)Phytochrome B (aa 1-908) linked to a trimer of ClpX (aa 61-425)
PB642(L-C)BBa_K365010 Version 1 (Component)Phytochrome B (aa 1-642) linked to a trimer of ClpX (aa 61-425)
BBa_K079026BBa_K079026 Version 1 (Component)GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter
BBa_J120027BBa_J120027 Version 1 (Component)It is a composite part containing CH1 domain of human antibody along with GFP.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
iGEM 2019 PlatesiGEM_2019_Plates Version 1 (Collection)384-well plates of dried DNA distributed by iGEM in Spring 2019
iGEM 2018 PlatesiGEM_2018_Plates Version 1 (Collection)384-well plates of dried DNA distributed by iGEM in Spring 2018
BBa_K1361006BBa_K1361006 Version 1 (Component)Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
BBa_K1607011BBa_K1607011 Version 1 (Component)The SCFV of anti-p185her2/neu antibody chA21 linked with EGFP by a (Gly4Ser)3 linker
BBa_M11051BBa_M11051 Version 1 (Component)Captures light energy and uses it to move protons across the membrane out of the cell.
BBa_K1172304BBa_K1172304 Version 1 (Component)Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS
BBa_K1968009BBa_K1968009 Version 1 (Component)PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
BBa_K1961000BBa_K1961000 Version 1 (Component)CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli
BBa_K1638030BBa_K1638030 Version 1 (Component)Leucine zipper fused to T25 domain of <i>cyaA</i> with cAMP-induced RFP generator
BBa_K1638031BBa_K1638031 Version 1 (Component)Leucine zipper fused to T18 domain of <i>cyaA</i> with cAMP-induced RFP generator
BBa_K2047001BBa_K2047001 Version 1 (Component)Fluorescent reporter system with stem-loop, GFP_Stem-loop with free energy of -25.6 kcal/mol
BBa_K1132037BBa_K1132037 Version 1 (Component)AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter
BBa_K1132038BBa_K1132038 Version 1 (Component)XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter
BBa_K2082231BBa_K2082231 Version 1 (Component)RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434
BBa_K1361002BBa_K1361002 Version 1 (Component)Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
BBa_K1412600BBa_K1412600 Version 1 (Component)Endow the CL-1(E.coli engineered strain) the ability of chemotaxis and quorum sensing
BBa_J120030BBa_J120030 Version 1 (Component)Composite part with Yeast promoter,CH1 domain of human Ab, ADAM17 cleavage sequence & Phoratoxin.
BBa_K1363604BBa_K1363604 Version 1 (Component)key of no of RNA logic gates
BBa_K1369010BBa_K1369010 Version 1 (Component)pBAD LamA-L-SH3pep, pLamA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations w
BBa_M45101BBa_M45101 Version 1 (Component)Composite biobrick intended to induce biofilm formation in the presence of lactose. Made from parts
LaLaLaBBa_K1479001 Version 1 (Component)0.0 Testing the UE of the bio-brick upload page, not designing a real one
BBa_K2055019BBa_K2055019 Version 1 (Component)Part designed for verification and characterization of the heat the shock promoter and Inverter.
BBa_K880001BBa_K880001 Version 1 (Component)Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K
BBa_K812132BBa_K812132 Version 1 (Component)mCFP with kozak sequence for expression in Xenopus