Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

lux pR
BBa_R0062 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pR)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

LasR+PAI
BBa_R0079 Version 1 (Component)
Promoter (LasR & PAI regulated)

BBa_K864400
BBa_K864400 Version 1 (Component)
Ptac, trp & lac regulated promoter

BBa_J33207
BBa_J33207 Version 1 (Component)
lac promoter and lacZ

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

RhlR+C4
BBa_R0071 Version 1 (Component)
Promoter (RhlR & C4-HSL regulated)

BBa_K1847014
BBa_K1847014 Version 1 (Component)
placUV5-lacO

lux pL
BBa_R0063 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pL)<br>

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_I13305
BBa_I13305 Version 1 (Component)
R0011.I13279 Lac-Regulated Las Amplifier

lacI
BBa_Q04121 Version 1 (Component)
QPI (B0034.C0012.B0015.R0011)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>

AraC
BBa_R0080 Version 1 (Component)
Promoter (AraC regulated)

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

cII p22
BBa_R0053 Version 1 (Component)
Promoter (p22 cII regulated)

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_I13307
BBa_I13307 Version 1 (Component)
R0011.I13277 Lac-Regulated Cin Amplifier

BBa_I13303
BBa_I13303 Version 1 (Component)
R0011.I13271 Lac-Regulated Rhl Amplifier

BBa_K180000
BBa_K180000 Version 1 (Component)
Hybrid promoter (trp & lac regulated -- tac pR)

lacZ-alpha
BBa_I732006 Version 1 (Component)
lacZ alpha fragment

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

cI 434
BBa_R0052 Version 1 (Component)
Promoter (434 cI regulated)

BBa_Q04510
BBa_Q04510 Version 1 (Component)
QPI (B0034.C0051.B0015.R0051)

pLac
BBa_K1638000 Version 1 (Component)
Lac operator

LacI
BBa_I732100 Version 1 (Component)
LacI

BBa_K731500
BBa_K731500 Version 1 (Component)
[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

BBa_I13306
BBa_I13306 Version 1 (Component)
R0040.I13279 Tet-Regulated Las Amplifier