BBa_K195403
BBa_K195403 Version 1 (Component)
This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv

BBa_J119300
BBa_J119300 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

BBa_K1947023
BBa_K1947023 Version 1 (Component)
This part serves as a catch system expressed in <i>E. coli.

BBa_K391001
BBa_K391001 Version 1 (Component)
Construct for characterization of P2 activity in the presence of AIP.

BBa_K391000
BBa_K391000 Version 1 (Component)
Construct for characterization of P2 activity in the presence of AIP.

BBa_I745007
BBa_I745007 Version 1 (Component)
TRE-Bak-IRES2-EGFP

GFP-AID
BBa_K812110 Version 1 (Component)
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

BBa_J119315
BBa_J119315 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A and B)

BBa_K078002
BBa_K078002 Version 1 (Component)
A fragment that encodes an enzyme involed in dioxin degradation, the third step

BBa_K1180002
BBa_K1180002 Version 1 (Component)
fusion protein of lamp 2b and RVG for brain targeting of the exosome

BBa_K1363005
BBa_K1363005 Version 1 (Component)
R0082-HfiA the HfiA protein express passway which can be regulated by light

BBa_K1963002
BBa_K1963002 Version 1 (Component)
A template for expression of sRNA fusions to the E. coli micC hairpin

BBa_K1551000
BBa_K1551000 Version 1 (Component)
To generate delta-4 fatty acid Desaturase so as to synthesize the DHA.

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K078004
BBa_K078004 Version 1 (Component)
2&#65292;2???&#65292;3-Trihydroxybiphenyl 1,2-dioxygenase. The second step enzyme in dixon degradati

pBAD-GFP
BBa_K845000 Version 1 (Component)
iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).

BBa_K1613015
BBa_K1613015 Version 1 (Component)
It is a new part which can detect cadmium ions in liquid.

BBa_K1613014
BBa_K1613014 Version 1 (Component)
It is a new part which can detect cadmium ions in liquid.

BBa_K1159019
BBa_K1159019 Version 1 (Component)
Membrane-anchored Human Protein Phosphatase 1 (PP1) in RFC[25] N-Part

BBa_K1159005
BBa_K1159005 Version 1 (Component)
Secretory Erythromycin Esterase Type II (IgKappa-SigP_EreB) in RFC[25] N-Part

BBa_J70032
BBa_J70032 Version 1 (Component)
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)

BBa_K1314011
BBa_K1314011 Version 1 (Component)
random sequence+T7 promoter+strong rbs+ amilCP, blue chromoprotein+B0015+part of mRFP

BBa_K1778000
BBa_K1778000 Version 1 (Component)
CYC1TATA sequence is derived from the TATA box secific sequence of yeast Saccharomyces cerevisiae

BBa_K1159108
BBa_K1159108 Version 1 (Component)
N-TEV-Protease_36AALinker_PIF6 (Second part for a light-switchable TEV-Protease) in RFC[25]

BBa_K1159107
BBa_K1159107 Version 1 (Component)
N-TEV-Protease_36AALinker_PIF3 (Second part for a light-switchable TEV-Protease) in RFC[25]

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

c-di-AMP
BBa_K1045002 Version 1 (Component)
CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch

BBa_K2122000
BBa_K2122000 Version 1 (Component)
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter

BBa_K1918102
BBa_K1918102 Version 1 (Component)
TRE-Kozak-EBFP2C(155-238)

BBa_K1918104
BBa_K1918104 Version 1 (Component)
TRE-Kozak-IntC:EBFP2C(155-238)

BBa_K1918101
BBa_K1918101 Version 1 (Component)
TRE-Kozak-EBFP2N(1-154)

BBa_K078005
BBa_K078005 Version 1 (Component)
2&#65292;2???&#65292;3-Trihydroxybiphenyl 1,2-dioxygenase. The second step enzyme in dixon degradati

BBa_K112212
BBa_K112212 Version 1 (Component)
{rbs.int!} The integrase gene with native rbs site and stop codon, BBb format

BBa_J48104
BBa_J48104 Version 1 (Component)
NikR promoter, a protein of the ribbon helix-helix family of trancription factors that repress expre

BBa_K855006
BBa_K855006 Version 1 (Component)
pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site

BBa_K855005
BBa_K855005 Version 1 (Component)
pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site

BBa_K1159313
BBa_K1159313 Version 1 (Component)
N-terminal <i>His₆</i>-tag in RFC[25] N-Part

BBa_K323080
BBa_K323080 Version 1 (Component)
CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system

BBa_K323029
BBa_K323029 Version 1 (Component)
CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system

GG100
BBa_K2145125 Version 1 (Component)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer

mdnA-myc-g
BBa_K627006 Version 1 (Component)
Fusion part of mdnA gene (from mdn-cluster) with myc-tag and gene III

BBa_K2170141
BBa_K2170141 Version 1 (Component)
Tetracycline inducible Outer membrane protein A signal peptide (TetR_TetO_OmpA) in RFC[25] N-part

GG98
BBa_K2145123 Version 1 (Component)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer

BBa_K182003
BBa_K182003 Version 1 (Component)
Intermediate part consisting of CI promoter followed by tetR, LacI and Tet Operator

BBa_K346089
BBa_K346089 Version 1 (Component)
T7 promoter(BBa_I719005)+MerT+MerP+MerC(the facilitation module of mercury absorption)

BBa_K896906
BBa_K896906 Version 1 (Component)
this is a hormone produced mainly in guts used for controlling the appetite

BBa_K737071
BBa_K737071 Version 1 (Component)
phoR, a histine kinase coding gene involved in phoBR regulon, is the regulatory protein that could a