SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_R4030BBa_R4030 Version 1 (Component)PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)
BBa_K1753000BBa_K1753000 Version 1 (Component)the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)
BBa_M31539BBa_M31539 Version 1 (Component)These are the genes in M13K07 responsible for forming the 'tip' of the phage.
BBa_K2151008BBa_K2151008 Version 1 (Component)p32-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site
BBa_K2151007BBa_K2151007 Version 1 (Component)p25-I13500: GFP under control of medium constitutive promoter and strong ribosome binding site
BBa_K2151006BBa_K2151006 Version 1 (Component)pHLBA-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site
BBa_K2082008BBa_K2082008 Version 1 (Component)Zika E-Protein: Fusion of Zika E-Protein with cI (434) regulatory protein with cMyc linker
BBa_K1758353BBa_K1758353 Version 1 (Component)Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promote
BBa_K1051801BBa_K1051801 Version 1 (Component)Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib
BBa_K1109000BBa_K1109000 Version 1 (Component)FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence with r
NsenseBBa_M36356 Version 1 (Component)A sensor that activates in the presence of nitrate in anaerobic conditions in E. coli.
BBa_K1638010BBa_K1638010 Version 1 (Component)T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> with GFP reporter
BBa_K1638009BBa_K1638009 Version 1 (Component)T18 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> with GFP reporter
BBa_K1159314BBa_K1159314 Version 1 (Component)TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part
BBa_K1114302BBa_K1114302 Version 1 (Component)This is <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_B001
Propane1BBa_K1655000 Version 1 (Component)Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.
BBa_M11411BBa_M11411 Version 1 (Component)Type 2 promoter of lrtA gene. Gene is expressed during darkness. Potentially darkness-induced promot
BBa_K1438010BBa_K1438010 Version 1 (Component)Bacterial Iron Storage Expressor
PhyB-908-(BBa_K365011 Version 1 (Component)Phytochrome B (aa 1-908) linked to a trimer of ClpX (aa 61-425)
PB642(L-C)BBa_K365010 Version 1 (Component)Phytochrome B (aa 1-642) linked to a trimer of ClpX (aa 61-425)
BBa_K079026BBa_K079026 Version 1 (Component)GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter
BBa_J120027BBa_J120027 Version 1 (Component)It is a composite part containing CH1 domain of human antibody along with GFP.
BBa_K079024BBa_K079024 Version 1 (Component)LacI repressor under the control of the J23118 costitutive promoter and Lac 1 operator
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
iGEM 2019 PlatesiGEM_2019_Plates Version 1 (Collection)384-well plates of dried DNA distributed by iGEM in Spring 2019
iGEM 2018 PlatesiGEM_2018_Plates Version 1 (Collection)384-well plates of dried DNA distributed by iGEM in Spring 2018
BBa_K1361006BBa_K1361006 Version 1 (Component)Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
BBa_J36849BBa_J36849 Version 1 (Component)Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag
BBa_J36851BBa_J36851 Version 1 (Component)Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag
BBa_K1607011BBa_K1607011 Version 1 (Component)The SCFV of anti-p185her2/neu antibody chA21 linked with EGFP by a (Gly4Ser)3 linker
BBa_M11051BBa_M11051 Version 1 (Component)Captures light energy and uses it to move protons across the membrane out of the cell.
BBa_K1172304BBa_K1172304 Version 1 (Component)Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS
BBa_K1961000BBa_K1961000 Version 1 (Component)CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli
BBa_K1638030BBa_K1638030 Version 1 (Component)Leucine zipper fused to T25 domain of <i>cyaA</i> with cAMP-induced RFP generator
BBa_K1638031BBa_K1638031 Version 1 (Component)Leucine zipper fused to T18 domain of <i>cyaA</i> with cAMP-induced RFP generator
BBa_K2047001BBa_K2047001 Version 1 (Component)Fluorescent reporter system with stem-loop, GFP_Stem-loop with free energy of -25.6 kcal/mol
BBa_K1132037BBa_K1132037 Version 1 (Component)AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter
BBa_K1132038BBa_K1132038 Version 1 (Component)XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter
BBa_K2082231BBa_K2082231 Version 1 (Component)RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434
BBa_K1361002BBa_K1361002 Version 1 (Component)Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
BBa_K1412600BBa_K1412600 Version 1 (Component)Endow the CL-1(E.coli engineered strain) the ability of chemotaxis and quorum sensing
BBa_J120030BBa_J120030 Version 1 (Component)Composite part with Yeast promoter,CH1 domain of human Ab, ADAM17 cleavage sequence & Phoratoxin.
BBa_K1363604BBa_K1363604 Version 1 (Component)key of no of RNA logic gates
BBa_K079021BBa_K079021 Version 1 (Component)LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La
BBa_M45101BBa_M45101 Version 1 (Component)Composite biobrick intended to induce biofilm formation in the presence of lactose. Made from parts
LaLaLaBBa_K1479001 Version 1 (Component)0.0 Testing the UE of the bio-brick upload page, not designing a real one
BBa_K2055019BBa_K2055019 Version 1 (Component)Part designed for verification and characterization of the heat the shock promoter and Inverter.
BBa_K880001BBa_K880001 Version 1 (Component)Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K
BBa_K812132BBa_K812132 Version 1 (Component)mCFP with kozak sequence for expression in Xenopus