BBa_K1886009BBa_K1886009 Version 1 (Component)AND GATE-output-2
BBa_K1886008BBa_K1886008 Version 1 (Component)AND GATE-output-1
BBa_K1172910BBa_K1172910 Version 1 (Component)Part 2 of the Biosafety-System L with Barnase (dplac Barnase)
BBa_K222002BBa_K222002 Version 1 (Component)project of a Mid1 gene disrupted from 489 to 2021 nt
BBa_K883503BBa_K883503 Version 1 (Component)TuYV coat protein with part of the readthrough with His-tag
BBa_K1641006BBa_K1641006 Version 1 (Component)Fusion protein of Vcre::EGFP::ssra-tag, with RBS at beginning
BBa_J70000BBa_J70000 Version 1 (Component)sbpa (a protein that forms crystals in the presence of calcium)
BBa_K1641007BBa_K1641007 Version 1 (Component)Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning
BBa_K1679019BBa_K1679019 Version 1 (Component)GFP+Promoter+RNA thermometer (FourU) +mRFP
BBa_K606062BBa_K606062 Version 1 (Component)pVeg SopVg T7 RNA amber TT
BBa_J23023BBa_J23023 Version 1 (Component)[Ptet][Key3][TT]
BBa_K1140006BBa_K1140006 Version 1 (Component)pTet + 37 oC RNA thermometer + mCherry (LVA)
BBa_K182100BBa_K182100 Version 1 (Component)Intermediate Part consisting of Lambda cI with Reporter Gene and double Terminators
BBa_K182002BBa_K182002 Version 1 (Component)Intermediate Part consisting of a cI regulated promoter and TetR (RBS+, Term-).
BBa_K180007BBa_K180007 Version 1 (Component)Game of Life - Secondary plasmid [tac pR, LuxI generator]
CBDclos(J6BBa_K863114 Version 1 (Component)CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
BBa_J34902BBa_J34902 Version 1 (Component)this part measures the happiness level of the cell
BBa_T9012BBa_T9012 Version 1 (Component)A mutant version of T9002 following a recombination event
BBa_I764000BBa_I764000 Version 1 (Component)production of tetR under the arabinose regulated promoter (AraC)
SARBBa_K2015000 Version 1 (Component)Self-assembling region (SAR) for the circularization of proteins
BBa_K078006BBa_K078006 Version 1 (Component)One part of the initial dioxygenase in dioxin degradation pathway
BBa_K1140002BBa_K1140002 Version 1 (Component)pLac + 32 oC RNA thermometer + GFP(LVA)
BBa_K1140003BBa_K1140003 Version 1 (Component)pCons + 37 oC RNA thermometer + LacI (LVA)
BBa_K1053102BBa_K1053102 Version 1 (Component)HHR*: HHR mutant type with RNA scaffold
BBa_K1160001BBa_K1160001 Version 1 (Component)chimeric RNA，combining crRNA and tracrRNA
BBa_K581003BBa_K581003 Version 1 (Component)SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)
rbs+ftsKBBa_I742164 Version 1 (Component)ftsK (cell division protein) of E. coli with native rbs
BBa_K2113010BBa_K2113010 Version 1 (Component)RNA thermometer and AMP with glycine
BBa_K2113009BBa_K2113009 Version 1 (Component)RNA thermometer and AMP without glycine
BBa_K1014014BBa_K1014014 Version 1 (Component)hrpL AND Gate device
epsE 5 ISBBa_K143005 Version 1 (Component)5??? Integration Sequence for the epsE locus of B. subtilis
epsE 3 ISBBa_K143006 Version 1 (Component)3??? Integration Sequence for the epsE locus of B. subtilis
BBa_K2056004BBa_K2056004 Version 1 (Component)NOx(Oxides of Nitrogen) sensor device (pNirK-Yellow chromoprotein)
BBa_K1641009BBa_K1641009 Version 1 (Component)Fusion protein of Vika-EGFP-ssra, with RBS at beginning
BBa_K833013BBa_K833013 Version 1 (Component)Lactate sensor derived from the AraF protein of E. coli
BBa_K786033BBa_K786033 Version 1 (Component)cascade proteins, which consists of casA, casB, casC, casD, casE
BBa_K563031BBa_K563031 Version 1 (Component)pEASY with first part of Tor2 without a original promoter
BBa_K779313BBa_K779313 Version 1 (Component)HHm-mKate - Repressible hammerhead attached to front of mKate MammoBlock
BBa_K1781006BBa_K1781006 Version 1 (Component)LZ-T18 - Leucine Zipper bound to T18 fragment of BACTH
CI-tagBBa_K1145007 Version 1 (Component)A protein which can inhibit the expression of Pλ.
BBa_K1648004BBa_K1648004 Version 1 (Component)Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane
Plac/ara-1BBa_K1145006 Version 1 (Component)Drive the expression of LuxRI in pLuxRI2 and its derivatives.
BBa_J120021BBa_J120021 Version 1 (Component)kappa 1 immunoglobulin light chain constant region of human antibody
PlsrBBa_K1145004 Version 1 (Component)Take effect when the density of bacteria hits certain value.
BBa_K1797014BBa_K1797014 Version 1 (Component)Gam and Bet-Two Components of the Red Recombination System
BBa_M45698BBa_M45698 Version 1 (Component)pCstA+RBS+amilGFP used to indication of low glucose concentration
BBa_K801005BBa_K801005 Version 1 (Component)RFC25 MCS for optional addition of C-terminal Strep-Tag
BBa_K1781005BBa_K1781005 Version 1 (Component)LZ-T25 - Leucine Zipper bound to T25 fragment of BACTH
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K1124117BBa_K1124117 Version 1 (Component)anti-csrA sRNA (+termintor) (sRNA generator)