Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

BBa_J61100
BBa_J61100 Version 1 (Component)
Ribosome Binding Site Family Member

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K212006
BBa_K212006 Version 1 (Component)
mRBP (modified ribose binding protein - now binds histamine)

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

LuxR
BBa_I0462 Version 1 (Component)
luxR Protein Generator

Prm +
BBa_I12006 Version 1 (Component)
Modified lamdba Prm promoter (repressed by 434 cI)

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_J61127
BBa_J61127 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_J61107
BBa_J61107 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_K1321009
BBa_K1321009 Version 1 (Component)
Nickel Binding Protein (NiBP) Freiburg format (RFC[25])

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K1151001
BBa_K1151001 Version 1 (Component)
Histidine-rich metal-binding protein

BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

BBa_K081014
BBa_K081014 Version 1 (Component)
RFP protein generator - PoPS->RFP

BBa_K1321002
BBa_K1321002 Version 1 (Component)
Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25

BBa_I13521
BBa_I13521 Version 1 (Component)
Ptet mRFP

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_K516030
BBa_K516030 Version 1 (Component)
mRFP protein generator TERM+ RBS+(B0030)

BBa_K118012
BBa_K118012 Version 1 (Component)
Synthetic ribosome binding site added by Son of Babel procedure

tetA(C)b
BBa_J31006 Version 1 (Component)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

BBa_K1321003
BBa_K1321003 Version 1 (Component)
Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25

BBa_K223000
BBa_K223000 Version 1 (Component)
SoxR binds with NO to activate SoxS (NO promoter)

BBa_J61117
BBa_J61117 Version 1 (Component)
Ribosome Binding Site Family Member

mRFP
BBa_K1615089 Version 1 (Component)
mRFP RFC25

BFP
BBa_K592100 Version 1 (Component)
Blue Fluorescent Protein (mTagBFP)

BBa_K115001
BBa_K115001 Version 1 (Component)
RNA thermometer (ROSE)

BBa_K228009
BBa_K228009 Version 1 (Component)
AraC protein(reversed sequence) and Pbad promoter

mRFP sel.
BBa_K1362422 Version 1 (Component)
mRFP selection marker with outward BsaI restriction sites