URA5
GenBank_CM000046_Region_302227_303005 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

URA5
GenBank_CM000046_Region_301512_302226 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

URA5
GenBank_CM000046_Region_303006_303540 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

Bs dif
BBa_K316002 Version 1 (Component)
dif excision site from B. subtilis

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_J61100
BBa_J61100 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_J85600
BBa_J85600 Version 1 (Component)
attB1 recombination site

Pveg-spoVG
BBa_K143053 Version 1 (Component)
Promoter Pveg and RBS spoVG for B. subtilis

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

Pveg
BBa_K143012 Version 1 (Component)
Promoter veg a constitutive promoter for B. subtilis

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

tetA(C)b
BBa_J31006 Version 1 (Component)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

RBS-GsiB
BBa_K143020 Version 1 (Component)
GsiB ribosome binding site (RBS) for B. subtilis

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_K209402
BBa_K209402 Version 1 (Component)
AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]

difF
BBa_I742101 Version 1 (Component)
dif site with forward orientation

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

difR
BBa_I742102 Version 1 (Component)
dif site with reverse orientation

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

amyE 5 IS
BBa_K143001 Version 1 (Component)
5??? Integration Sequence for the amyE locus of B. subtilis