Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

ssrA(DAS+4
BBa_K1075020 Version 1 (Component)
E. coli ssrA(DAS+4)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K1075021
BBa_K1075021 Version 1 (Component)
E. coli ssrA(DAS+4)-TT

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_I746200
BBa_I746200 Version 1 (Component)
FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

BBa_K1399007
BBa_K1399007 Version 1 (Component)
GFP (mut3b) with SsrA-DAS+2 degradation tag

BBa_K1075041
BBa_K1075041 Version 1 (Component)
RBS32-MazE-(Ec)ssrA(DAS+4)

BBa_K1075024
BBa_K1075024 Version 1 (Component)
RBS34- mCherry- (Ec)ssrA(DAS+4)-TT

BBa_K1399008
BBa_K1399008 Version 1 (Component)
GFP (mut3b) with DAS-ssrA degradation tag

BBa_K912001
BBa_K912001 Version 1 (Component)
E. coli phytase (appa phytase)

sacB
BBa_K322921 Version 1 (Component)
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.

BBa_K1075023
BBa_K1075023 Version 1 (Component)
mCherry- (Ec)ssrA(DAS+4)-TT

BBa_K1075022
BBa_K1075022 Version 1 (Component)
mCherry- (Ec)ssrA(DAS+4)

TEVsite
BBa_J18918 Version 1 (Component)
TEV cleavage site (E. coli)

BBa_K1075025
BBa_K1075025 Version 1 (Component)
pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT

BBa_K953004
BBa_K953004 Version 1 (Component)
Hemeoxygenase with RBS (E. coli Codon Optimised)

BBa_K1172901
BBa_K1172901 Version 1 (Component)
Alanine racemase from ''E. coli''

dxs
BBa_K118000 Version 1 (Component)
dxs coding sequence encoding 1-deoxyxylulose-5-phosphate synthase

BBa_K1075043
BBa_K1075043 Version 1 (Component)
pLac-RBS32-mazE-(Ec)ssrA(DAS+4)-TT

BBa_K863006
BBa_K863006 Version 1 (Component)
ecol laccase from E. coli

BBa_J70024
BBa_J70024 Version 1 (Component)
TetM gene, recoded compromise E. coli & Mesoplasma florum

BBa_K1075042
BBa_K1075042 Version 1 (Component)
RBS32-MazE-(Ec)ssrA(DAS+4)-TT

BBa_M1716
BBa_M1716 Version 1 (Component)
Hepcidin Optimized for E. coli

BBa_K523001
BBa_K523001 Version 1 (Component)
RBS + malS (E. coli periplasmic α-amylase)

BBa_K523002
BBa_K523002 Version 1 (Component)
RBS + bglX (E. coli perisplasmic β-glucosidase)

BBa_K953002
BBa_K953002 Version 1 (Component)
Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)