BBa_K1412829
BBa_K1412829 Version 1 (Component)
Characterize efficiency of RBS with chemotaxis

Ag43+FLAG
BBa_K1352011 Version 1 (Component)
Ag43+FLAG+MCS for surface display of foreign peptides

BBa_K1316020
BBa_K1316020 Version 1 (Component)
2nd part of Constitutive Anderson promoter + csgA:Histag curli gene

BBa_K1316019
BBa_K1316019 Version 1 (Component)
2nd part of Constitutive Anderson promoter + csgA curli gene

BBa_K1796015
BBa_K1796015 Version 1 (Component)
complete line of nif cluster

BBa_K1638005
BBa_K1638005 Version 1 (Component)
T18 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> (IPTG inducible)

SP-aprE
BBa_K1674000 Version 1 (Component)
Secretion signal peptide originated from the B.subtilis extracellular protease AprE

BBa_M11082
BBa_M11082 Version 1 (Component)
ZntR:Zn(II)-responsive regulator of zntA (also MerR transcriptional regulator)

BBa_K1159101
BBa_K1159101 Version 1 (Component)
N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

sRIP
BBa_K237003 Version 1 (Component)
RIP +export signal (OmpA). Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA)

BBa_K137115
BBa_K137115 Version 1 (Component)
Constitutively expresses cI, the lambda repressor.

BBa_J64903
BBa_J64903 Version 1 (Component)
Light induced production of RNAi for glnG

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_M36529
BBa_M36529 Version 1 (Component)
In presence of lactose, produces mCherry

BBa_K077042
BBa_K077042 Version 1 (Component)
cII under control of plac promotor

BBa_K648001
BBa_K648001 Version 1 (Component)
cI repressor under control of constitutive

BBa_K2163000
BBa_K2163000 Version 1 (Component)
Measurement of Protease Activity using FRET

BBa_K077040
BBa_K077040 Version 1 (Component)
cII under control of plac promotor

BBa_K091120
BBa_K091120 Version 1 (Component)
pTet regulated production of LacI protein

BBa_I739013
BBa_I739013 Version 1 (Component)
Composite of BBa_I739001, BBa_I739002 and BBa_I739003

BBa_J107051
BBa_J107051 Version 1 (Component)
Constitutive generator of Beta-Galactosidase + J23100

BBa_K1403018
BBa_K1403018 Version 1 (Component)
Expression of agaA in E coli

BBa_J23068
BBa_J23068 Version 1 (Component)
Intermediate #2 in construction of TraGr cassette

BBa_K2027014
BBa_K2027014 Version 1 (Component)
Mutant Feedback-Resistant Version of AroG (Leu175Asp)

BBa_K216017
BBa_K216017 Version 1 (Component)
luxCDE genes of Xenorhabdus luminescens (aldehyde biosynthesis)

BBa_K1919102
BBa_K1919102 Version 1 (Component)
Mutant type cIts repressor of device BBa_K1919100

BBa_J119407
BBa_J119407 Version 1 (Component)
Pupp promoter mutant - Deletion of -10 element

BBa_K1935009
BBa_K1935009 Version 1 (Component)
pMYO-3 : Specific muscle promoter of C.elegans

BBa_K2027013
BBa_K2027013 Version 1 (Component)
Mutant Feedback-Resistant Version of AroG (Pro150Leu)

BBa_M31512
BBa_M31512 Version 1 (Component)
section of M13.1 (HpaI to BamHI section)

BBa_K323012
BBa_K323012 Version 1 (Component)
C-split fragment of mCerulean with ATG

BBa_K1401006
BBa_K1401006 Version 1 (Component)
MoClo Level 0 part of L3S2P21 terminator

BBa_K1459002
BBa_K1459002 Version 1 (Component)
C-term of PmrB from Salmonella enterica

BBa_M31901
BBa_M31901 Version 1 (Component)
HpaI to BamHI section of M13K07 genome

fcs nickna
BBa_I750003 Version 1 (Component)
a short description of this part fcs

BBa_K323055
BBa_K323055 Version 1 (Component)
C-split fragment of mCitrine with ATG

BBa_K2027012
BBa_K2027012 Version 1 (Component)
Mutant Feedback-Resistant Version of MelA Tyrosinase

BBa_K418007
BBa_K418007 Version 1 (Component)
chitin synthase (CHS3) gene of S. cerevisiae

BBa_K1401005
BBa_K1401005 Version 1 (Component)
MoClo Level 0 part of ECK120015170 terminator

BBa_K812059
BBa_K812059 Version 1 (Component)
A GoldenBricked version of week RBS J61117

BBa_M36025
BBa_M36025 Version 1 (Component)
phiX174 gene B - mutated rbs of K

BBa_J100173
BBa_J100173 Version 1 (Component)
Designed to optimize testing of fitness genes

BBa_K2027010
BBa_K2027010 Version 1 (Component)
Repetitive Insertion of 4D Melanin-Binding Heptapeptide

BBa_K189103
BBa_K189103 Version 1 (Component)
Genome of GenSniper via Top-Down approach

BBa_M31514
BBa_M31514 Version 1 (Component)
Refactored HpaI to BamHI region of M13K07

BBa_J22146
BBa_J22146 Version 1 (Component)
The Lac Y and mRFP gene under the RecA(SOS) promoter.

BBa_I712017
BBa_I712017 Version 1 (Component)
Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.

BBa_K774102
BBa_K774102 Version 1 (Component)
Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin

BBa_I744003
BBa_I744003 Version 1 (Component)
protein generator for biosynthesis of vanillin

BBa_M31530
BBa_M31530 Version 1 (Component)
Refactored Portion of M13K07 Genome