BBa_K1172910
BBa_K1172910 Version 1 (Component)
Part 2 of the Biosafety-System L with Barnase (dplac Barnase)

BBa_K222002
BBa_K222002 Version 1 (Component)
project of a Mid1 gene disrupted from 489 to 2021 nt

BBa_K883503
BBa_K883503 Version 1 (Component)
TuYV coat protein with part of the readthrough with His-tag

BBa_K1641006
BBa_K1641006 Version 1 (Component)
Fusion protein of Vcre::EGFP::ssra-tag, with RBS at beginning

BBa_J70000
BBa_J70000 Version 1 (Component)
sbpa (a protein that forms crystals in the presence of calcium)

BBa_K1641007
BBa_K1641007 Version 1 (Component)
Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning

BBa_K182100
BBa_K182100 Version 1 (Component)
Intermediate Part consisting of Lambda cI with Reporter Gene and double Terminators

BBa_K182002
BBa_K182002 Version 1 (Component)
Intermediate Part consisting of a cI regulated promoter and TetR (RBS+, Term-).

BBa_K180007
BBa_K180007 Version 1 (Component)
Game of Life - Secondary plasmid [tac pR, LuxI generator]

CBDclos(J6
BBa_K863114 Version 1 (Component)
CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP

BBa_T9012
BBa_T9012 Version 1 (Component)
A mutant version of T9002 following a recombination event

BBa_I764000
BBa_I764000 Version 1 (Component)
production of tetR under the arabinose regulated promoter (AraC)

SAR
BBa_K2015000 Version 1 (Component)
Self-assembling region (SAR) for the circularization of proteins

BBa_J70556
BBa_J70556 Version 1 (Component)
BsaXI test circuit: J70555.J70554 or J70554.J70550.J70554

BBa_K078006
BBa_K078006 Version 1 (Component)
One part of the initial dioxygenase in dioxin degradation pathway

rbs+ftsK
BBa_I742164 Version 1 (Component)
ftsK (cell division protein) of E. coli with native rbs

BBa_J17000
BBa_J17000 Version 1 (Component)
G-alpha-olf

epsE 5 IS
BBa_K143005 Version 1 (Component)
5??? Integration Sequence for the epsE locus of B. subtilis

epsE 3 IS
BBa_K143006 Version 1 (Component)
3??? Integration Sequence for the epsE locus of B. subtilis

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2056004
BBa_K2056004 Version 1 (Component)
NOx(Oxides of Nitrogen) sensor device (pNirK-Yellow chromoprotein)

BBa_K1641009
BBa_K1641009 Version 1 (Component)
Fusion protein of Vika-EGFP-ssra, with RBS at beginning

BBa_K833013
BBa_K833013 Version 1 (Component)
Lactate sensor derived from the AraF protein of E. coli

BBa_K786033
BBa_K786033 Version 1 (Component)
cascade proteins, which consists of casA, casB, casC, casD, casE

BBa_K563031
BBa_K563031 Version 1 (Component)
pEASY with first part of Tor2 without a original promoter

BBa_K779313
BBa_K779313 Version 1 (Component)
HHm-mKate - Repressible hammerhead attached to front of mKate MammoBlock

BBa_K1781006
BBa_K1781006 Version 1 (Component)
LZ-T18 - Leucine Zipper bound to T18 fragment of BACTH

CI-tag
BBa_K1145007 Version 1 (Component)
A protein which can inhibit the expression of Pλ.

BBa_K1648004
BBa_K1648004 Version 1 (Component)
Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane

Plac/ara-1
BBa_K1145006 Version 1 (Component)
Drive the expression of LuxRI in pLuxRI2 and its derivatives.

BBa_J120021
BBa_J120021 Version 1 (Component)
kappa 1 immunoglobulin light chain constant region of human antibody

Plsr
BBa_K1145004 Version 1 (Component)
Take effect when the density of bacteria hits certain value.

BBa_K1797014
BBa_K1797014 Version 1 (Component)
Gam and Bet-Two Components of the Red Recombination System

BBa_M45698
BBa_M45698 Version 1 (Component)
pCstA+RBS+amilGFP used to indication of low glucose concentration

BBa_K801005
BBa_K801005 Version 1 (Component)
RFC25 MCS for optional addition of C-terminal Strep-Tag

BBa_K1781005
BBa_K1781005 Version 1 (Component)
LZ-T25 - Leucine Zipper bound to T25 fragment of BACTH

BBa_K101003
BBa_K101003 Version 1 (Component)
Construct of TetR promoter, RBS, Lambda cI gene, terminator

BBa_K891008
BBa_K891008 Version 1 (Component)
Probe coding for alpha fragment of beta gal 2

BBa_K218017
BBa_K218017 Version 1 (Component)
LuxO D47E under constitutive expression of TetR repressible promoter

BBa_K891004
BBa_K891004 Version 1 (Component)
Probe coding for the Omega Fragment of beta galactosidase

BBa_K2041020
BBa_K2041020 Version 1 (Component)
promoter-RBS-binding site of miR-155-tetR-T

BBa_M50051
BBa_M50051 Version 1 (Component)
IPTG Inducible Plasmid for the Expression of LsrR Repressor

BBa_K218021
BBa_K218021 Version 1 (Component)
LuxO D47A under constitutive expression of tetR repressible promoter

BBa_K1472611
BBa_K1472611 Version 1 (Component)
IPTG inducible expression of ∆9, ∆12, and ∆15 desaturases

BBa_K101004
BBa_K101004 Version 1 (Component)
Construct of LacI promoter, RBS, p22 mnt gene, terminator

BBa_K2039000
BBa_K2039000 Version 1 (Component)
Part expressing the dimerization protein FKBP and GFP10 (subunit of tripartite GFP)

BBa_K2039001
BBa_K2039001 Version 1 (Component)
Part expressing the dimerization protein FRB* and GFP11 (subunit of tripartite GFP)

CBDclos(T7
BBa_K863112 Version 1 (Component)
Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K748000
BBa_K748000 Version 1 (Component)
AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.

BBa_K524203
BBa_K524203 Version 1 (Component)
pSC101 heat sensitive origin of replication with deletion in par locus