iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
BBa_B0034BBa_B0034 Version 1 (Component)RBS (Elowitz 1999) -- defines RBS efficiency
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
mCherryBBa_J06504 Version 1 (Component)monomeric RFP optimized for bacteria
luxIBBa_C0061 Version 1 (Component)autoinducer synthetase for AHL
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
araCBBa_C0080 Version 1 (Component)araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
BBa_J70029BBa_J70029 Version 1 (Component)RBS + mCherry gene, optimized for expression in Me. florum and E. coli
BBa_K953004BBa_K953004 Version 1 (Component)Hemeoxygenase with RBS (E. coli Codon Optimised)
HisTagBBa_J18909 Version 1 (Component)His tag (6xHis; E. coli - optimized)
BBa_K953002BBa_K953002 Version 1 (Component)Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)
FurBO_11498 Version 1 (Component) pT7BBa_K525998 Version 1 (Component)Promoter T7 and RBS
RBS-RFPBBa_K093005 Version 1 (Component)RFP with RBS
BBa_K953000BBa_K953000 Version 1 (Component)Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
InteinBBa_K300004 Version 1 (Component)Engineered pH-inducible intein (codon optimized for E. coli) - internal domain
BBa_K1550010BBa_K1550010 Version 1 (Component)BmtA (metallothionein) codon optimized for E. coli, with RBS and tt
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
preSCsiteBBa_J18919 Version 1 (Component)preScission protease cleavage site (E. coli-optimized)
mCherryBBa_J06505 Version 1 (Component)monomeric RFP optimized for bacteria
T7 RBSBBa_K1362090 Version 1 (Component)strong T7 RBS
BBa_K1550004BBa_K1550004 Version 1 (Component)BmtA (Lead/Zinc-binding metallothionein from Oscillatoria brevis, codon optimized for E. coli)
BBa_J33201BBa_J33201 Version 1 (Component)E. coli chromosomal ars promoter with arsR repressor gene
BBa_K1319004BBa_K1319004 Version 1 (Component)TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli
BBa_M1716BBa_M1716 Version 1 (Component)Hepcidin Optimized for E. coli
BBa_I746200BBa_I746200 Version 1 (Component)FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
scarBBa_G0000 Version 1 (Component)SpeI/XbaI scar for RBS-CDS junctions
adhBBBa_K173017 Version 1 (Component)alcohol dehydrogenase II from Z. mobilis (codon optimized for E. coli)
BBa_C0261BBa_C0261 Version 1 (Component)AHL-making Enzyme, luxI (+RBS)
PelBBBa_J32015 Version 1 (Component)PelB leader sequence; directs protein to E. coli periplasmic membrane
BBa_K1321011BBa_K1321011 Version 1 (Component)SmtA metallothionein in Freiburg format (RFC[25])
BBa_K880005BBa_K880005 Version 1 (Component)Strong promoter, strong RBS combination for high expression levels of proteins