pSBBs1C
BBa_K823021 Version 1 (Component)
pSB_Bs1C-<i>lacZ</i> (lacZ reporter vector for <i>B. subtilis</i>)

BBa_K1739003
BBa_K1739003 Version 1 (Component)
Sequence coding for Envirowire: Sup35NM with N-terminal CsgAss and C-terminal cytb562

BBa_K1711002
BBa_K1711002 Version 1 (Component)
yeVenus-PEST-TheoA; Coding sequence for destabilized YFP Venus and theophylline-sensitive aptazyme

BBa_K1088053
BBa_K1088053 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_J119315
BBa_J119315 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A and B)

BBa_M36111
BBa_M36111 Version 1 (Component)
Lysis gene E for phiX174, synonymous mutation on gene J's rbs inside E

BBa_K1180002
BBa_K1180002 Version 1 (Component)
fusion protein of lamp 2b and RVG for brain targeting of the exosome

pSB1C3 GB
BBa_K812050 Version 1 (Component)
Golden brick version of pSB1C3 plasmid for Golden Gate and Golden Brick cloning

BBa_K1711001
BBa_K1711001 Version 1 (Component)
yeVenus-TheoA; coding sequence for enhanced yellow fluorescent protein and theophylline-sensative ap

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K351004
BBa_K351004 Version 1 (Component)
Modified Human FGF receptor for MPT51 antigen with fission yeast surface signal peptide

BBa_K1446001
BBa_K1446001 Version 1 (Component)
dcmA-gene with N-terminal pdu-microcompartment tag, codon optimised for E.coli (K12)

pTetR-LacI
BBa_I763030 Version 1 (Component)
LacI coding device regulated by pTetR

BBa_K660605
BBa_K660605 Version 1 (Component)
OmpF + wYFP + TT

BBa_K1124109
BBa_K1124109 Version 1 (Component)
pLac-mCherry-GFP-pLambda-sRNA(anti-mCherry) (for sRNA mechanism check)

BBa_K415023
BBa_K415023 Version 1 (Component)
pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Plux/cI-OR:LuxI

pSBBs2E
BBa_K823027 Version 1 (Component)
pSB_Bs2E: Empty backbone for integration into Bacillus subtilis lacA locus

BBa_K101009
BBa_K101009 Version 1 (Component)
Dual-Repressed Promoter for p22 Mnt and TetR with RBS (med strength)

BBa_K606068
BBa_K606068 Version 1 (Component)
pVeg SpoVG cI SpoVG RFP Term for B. subtilis and E. coli

Switch
BBa_K1185005 Version 1 (Component)
E. coli L-form Switch

BBa_K299509
BBa_K299509 Version 1 (Component)
Expression Vector pT7+B0034

BBa_I2049
BBa_I2049 Version 1 (Component)
mCherry & GFP expression system

BBa_K549039
BBa_K549039 Version 1 (Component)
fur is an iron-dependent regulator from N. meningitidis

BBa_M36663
BBa_M36663 Version 1 (Component)
fur binding site, PopS repressor

BBa_K243028
BBa_K243028 Version 1 (Component)
FOS-Split Linker-Fok_a

BBa_M36689
BBa_M36689 Version 1 (Component)
HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB site + CMV Reverse + attP site

BBa_K1159117
BBa_K1159117 Version 1 (Component)
Red light triggered Kill Switch for plants translation unit (PhyB/PIF3 version)

BBa_K1648003
BBa_K1648003 Version 1 (Component)
Recombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator

BBa_K1159118
BBa_K1159118 Version 1 (Component)
Red light triggered Kill Switch for plants translation unit (PhyB/PIF6 version)

BBa_J70032
BBa_J70032 Version 1 (Component)
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)

BBa_M33334
BBa_M33334 Version 1 (Component)
CDS+RBS for Inorganic mercury-sensitive regulatory protein merR coding sequence with RBS

BBa_K547000
BBa_K547000 Version 1 (Component)
ready-to-inject backbone for T3SS, SlrP taged, with Bsa I cloning site

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1326007
BBa_K1326007 Version 1 (Component)
Plac + POR + DVR1 + ChlP

BBa_K1159108
BBa_K1159108 Version 1 (Component)
N-TEV-Protease_36AALinker_PIF6 (Second part for a light-switchable TEV-Protease) in RFC[25]

BBa_K1159107
BBa_K1159107 Version 1 (Component)
N-TEV-Protease_36AALinker_PIF3 (Second part for a light-switchable TEV-Protease) in RFC[25]

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

BBa_K1113401
BBa_K1113401 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K299029
BBa_K299029 Version 1 (Component)
RBS measurement device pT7 J61127 GFP

BBa_K782048
BBa_K782048 Version 1 (Component)
HindIII/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K782050
BBa_K782050 Version 1 (Component)
BamHI/XbaI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782049
BBa_K782049 Version 1 (Component)
HindIII/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K782051
BBa_K782051 Version 1 (Component)
BamHI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782052
BBa_K782052 Version 1 (Component)
BamHI/SacI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782058
BBa_K782058 Version 1 (Component)
SacI/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K1180003
BBa_K1180003 Version 1 (Component)
Fusion protein of lamp 2b and pre-s1 for liver targeting of the exosome

BBa_M36745
BBa_M36745 Version 1 (Component)
The sensor codes for AraC, a transcription factor, which represses the operon.Arabinose induces tran

BBa_K782044
BBa_K782044 Version 1 (Component)
EcoRI/XbaI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782057
BBa_K782057 Version 1 (Component)
SacI/SpeI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors

BBa_K782043
BBa_K782043 Version 1 (Component)
EcoRI/BamHI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors