pSB1A3 lac
BBa_K627015 Version 1 (Component)
Plasmid backbone pSB1A3 carrying the lac operon induction system

BBa_K592001
BBa_K592001 Version 1 (Component)
CcaS, green light sensor from Synechocystis sp. PCC6803

BBa_K592016
BBa_K592016 Version 1 (Component)
B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS

BBa_K592019
BBa_K592019 Version 1 (Component)
PcpcG2-B0034-amilGFP green light sensor output

BBa_K592020
BBa_K592020 Version 1 (Component)
PFixK2-lambda C1-B0034-amilCP blue light sensor output

BBa_K539711
BBa_K539711 Version 1 (Component)
ilvC (ketol-acid reductoisomerase NAD(P)-binding)

BBa_K615000
BBa_K615000 Version 1 (Component)
E. coli strain for His3-URA3 one-hybrid selection system

BBa_K592029
BBa_K592029 Version 1 (Component)
PcpcG2-B0034-BFP green light sensor output

BBa_K592030
BBa_K592030 Version 1 (Component)
PcpcG2-B0034-YFP green light sensor output

BBa_K592201
BBa_K592201 Version 1 (Component)
Low to medium copy Lambda Red recombineering compatible plasmid

K592202
BBa_K592202 Version 1 (Component)
Low to medium copy Lambda Red recombineering compatible plasmid

BBa_K560000
BBa_K560000 Version 1 (Component)
Blue Light Sensor+PtrpL+mRFP1

BBa_K566005
BBa_K566005 Version 1 (Component)
pCpcG2 promoter (Green light inducible)

BBa_K597100
BBa_K597100 Version 1 (Component)
avi-RFP [AviTagged red fluorescent protein]

BBa_K535003
BBa_K535003 Version 1 (Component)
FeOx -> Clostridium acetobutylicum???s ferredoxin

BBa_K535005
BBa_K535005 Version 1 (Component)
HydA1 - FeOx construction (Hydrogenase-ferredoxin fussion)

BBa_K535006
BBa_K535006 Version 1 (Component)
PFOR -> D. africanus Pyruvate-Ferredoxin OxidoReductase

BBa_K615003
BBa_K615003 Version 1 (Component)
E. coli strain for TolC one-hybrid selection system

pCpcG2 inv
BBa_K566009 Version 1 (Component)
pCpcG2 promoter, inverted sequence (Green light inducible)

BBa_K654060
BBa_K654060 Version 1 (Component)
Fatty Acyl-CoA Reductase with 8-His Tag

BBa_K654062
BBa_K654062 Version 1 (Component)
Fatty Acyl ACP Reductase with 8-His Tag

BBa_K566018
BBa_K566018 Version 1 (Component)
E. coli MXRed

BBa_K566019
BBa_K566019 Version 1 (Component)
E. coli MxRed

Cph8 gene
BBa_K566026 Version 1 (Component)
Cph8 gene for red-photoreceptor (constitutive expression)

BBa_K654066
BBa_K654066 Version 1 (Component)
Fatty Acyl-CoA Reductase + Terminator

BBa_K654068
BBa_K654068 Version 1 (Component)
Fatty ACP Reductase + Terminator

BBa_K619889
BBa_K619889 Version 1 (Component)
prfA-UTR, RNA thermosensor from Listeria monocytogenes

BBa_K748000
BBa_K748000 Version 1 (Component)
AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.

BBa_K748001
BBa_K748001 Version 1 (Component)
AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.

BBa_K654074
BBa_K654074 Version 1 (Component)
psbA2 controlled Fatty ACP Reductase

BBa_K896000
BBa_K896000 Version 1 (Component)
SQR(sulfide quinone reductase),from Synechococcus sp. PCC 7002 plasmid pAQ7

BBa_K823051
BBa_K823051 Version 1 (Component)
mKate2, a red monomeric fluorescent protein + RBS + Term

BBa_K936017
BBa_K936017 Version 1 (Component)
K206000+B0034+Reductase (anaerobic)+B0034+Dehydrogenase

BBa_K936018
BBa_K936018 Version 1 (Component)
B0034+Reductase+B0015

BBa_K896003
BBa_K896003 Version 1 (Component)
Sulfide quinone reductase(SQR) on neutral site 2 vector

BBa_K812013
BBa_K812013 Version 1 (Component)
GFP-AID OsTirI polysistronic system for auxin detection in tadpole

BBa_K897318
BBa_K897318 Version 1 (Component)
Paired termini structure

TBY
BBa_K897720 Version 1 (Component)
Antisense FtsZ protected by paired termini structure under T7 promoter

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K759005
BBa_K759005 Version 1 (Component)
phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K1982002
BBa_K1982002 Version 1 (Component)
Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)

BBa_K759007
BBa_K759007 Version 1 (Component)
RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

rTT+pR
BBa_K093003 Version 1 (Component)
Convergent Promoter System: Forward Module: rTT+lambda pR