BBa_K112217
BBa_K112217 Version 1 (Component)
{a~ihfA!} The ihf alpha gene ready to have rbs attached with stop codon, BBb format

BBa_K112223
BBa_K112223 Version 1 (Component)
{a~ihfB!} The ihf beta gene ready to have rbs attached and stop codon, BBb format

BBa_K112211
BBa_K112211 Version 1 (Component)
{a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format

BBa_K1655001
BBa_K1655001 Version 1 (Component)
This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.

BBa_K880001
BBa_K880001 Version 1 (Component)
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K

BBa_S03635
BBa_S03635 Version 1 (Component)
HixC-pBad-HixC-RBS-TetF : HixC-TT-RE

BBa_K2176001
BBa_K2176001 Version 1 (Component)
Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A

BBa_K1216014
BBa_K1216014 Version 1 (Component)
BBa_K1216014 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216012
BBa_K1216012 Version 1 (Component)
BBa_K1216012 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216013
BBa_K1216013 Version 1 (Component)
BBa_K1216013 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216011
BBa_K1216011 Version 1 (Component)
BBa_K1216007 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216010
BBa_K1216010 Version 1 (Component)
BBa_K1216010 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL. (

xis
BBa_K112204 Version 1 (Component)
{a~xis!} The bacteriophage lambda xis gene ready to have rbs attached and stop codon; assembly stand

BBa_K325109
BBa_K325109 Version 1 (Component)
EPIC Firefly Luciferase and LRE (under pBAD)<BR><i>P. Pyralis<BR>(E. coli optimised)</i>

BBa_K1361004
BBa_K1361004 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag

BBa_K2082237
BBa_K2082237 Version 1 (Component)
RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein

BBa_K1113501
BBa_K1113501 Version 1 (Component)
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag

BBa_K1053012
BBa_K1053012 Version 1 (Component)
taRNA(taR12y) responsive Hammerhead Ribozyme(TR(12y)-HHR) with RBS

BBa_K1053011
BBa_K1053011 Version 1 (Component)
taRNA(taR42) responsive Hammerhead Ribozyme(TR(42)-HHR) with RBS

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_I716212
BBa_I716212 Version 1 (Component)
Cre (GTG start)

BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein

BBa_K2150029
BBa_K2150029 Version 1 (Component)
pCon+RBS+tetR+DT+pTet+RBS+T7RNAP+Ter+RBS+pT7+tetX-GFP(fusion protein)+DT

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

BBa_K187416
BBa_K187416 Version 1 (Component)
trpE in pAB

Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')

BBa_K327013
BBa_K327013 Version 1 (Component)
The initiator

BBa_J3106
BBa_J3106 Version 1 (Component)
pBad-HixC-TetB-RBS_rev-HixC-TT-RE

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K594015
BBa_K594015 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

BBa_I715065
BBa_I715065 Version 1 (Component)
Burnt Pancake Tester with RE and backwards Tet resistance

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K1361003
BBa_K1361003 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_K1361001
BBa_K1361001 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively weak expression of CsgA,C

BBa_J58009
BBa_J58009 Version 1 (Component)
Fusion protein Trg-EnvZ

BBa_K2044000
BBa_K2044000 Version 1 (Component)
Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8

BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_J44008
BBa_J44008 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44006
BBa_J44006 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE