BBa_K758004
BBa_K758004 Version 1 (Component)
GAL4, this part coding protein binds and activates the UAS sequence

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K737016
BBa_K737016 Version 1 (Component)
This part conteins gvpA protein's coding sequence.

BBa_K737017
BBa_K737017 Version 1 (Component)
This part conteins gvpC-20psi protein

BBa_K737011
BBa_K737011 Version 1 (Component)
gvpC-GFP fusion protein with medium constitutive promoter

BBa_K737012
BBa_K737012 Version 1 (Component)
gvpC-GFP fusion protein UH with medium constitutive promoter

BBa_K737013
BBa_K737013 Version 1 (Component)
gvpC-GFP fusion protein

BBa_K737014
BBa_K737014 Version 1 (Component)
gvpC-GFP fusion protein UH

BBa_J72210
BBa_J72210 Version 1 (Component)
Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site

BBa_K737037
BBa_K737037 Version 1 (Component)
J23106,P0412,R0011,ClaI site,SalI site,truncated Spot42

CBDcex
BBa_K863101 Version 1 (Component)
Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)

BBa_K737057
BBa_K737057 Version 1 (Component)
Spot42 have a weak Hfq binding site(whereas galK weaker),an endogenous terminator(unclear efficiency

CBDclos
BBa_K863111 Version 1 (Component)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)

BBa_K873000
BBa_K873000 Version 1 (Component)
heat shock 10kDa protein(hsp 10) groES coding resquence

BBa_K739004
BBa_K739004 Version 1 (Component)
Ax21 protein - signaling part

BBa_K737024
BBa_K737024 Version 1 (Component)
PhoB promotor ligated to GFP

BBa_K737038
BBa_K737038 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by IPTG

BBa_K737039
BBa_K737039 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by aTc

BBa_K737040
BBa_K737040 Version 1 (Component)
galK::GFP generator ligated to TetR generator and constitutive sRNA device

BBa_K737041
BBa_K737041 Version 1 (Component)
galK::GFP generator ligated to LacI generator and constitutive sRNA device

BBa_K737042
BBa_K737042 Version 1 (Component)
constitutive Spot42 generator ligated to TetR generator

BBa_K737043
BBa_K737043 Version 1 (Component)
constitutive Spot42 generator ligated to lacI generator

BBa_K737053
BBa_K737053 Version 1 (Component)
Plac-GFP Generator ligated to TetR Generator

BBa_K737054
BBa_K737054 Version 1 (Component)
Ptet-GFP Generator ligated to LacI Generator

BBa_K782004
BBa_K782004 Version 1 (Component)
TALA:NLS DNA binding domain

BBa_K782005
BBa_K782005 Version 1 (Component)
TALD:NLS DNA binding domain

BBa_K782006
BBa_K782006 Version 1 (Component)
TALB:NLS DNA binding domain

BBa_K782007
BBa_K782007 Version 1 (Component)
NicTAL12:NLS DNA binding domain

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

pLac ColE2
BBa_K914001 Version 1 (Component)
pLac Colicin E2 Immunity protein

BBa_K914002
BBa_K914002 Version 1 (Component)
repressilator RBS Colicin E2 Immunity protein

BBa_K883160
BBa_K883160 Version 1 (Component)
CCMV_Negative coat protein

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

BBa_K157004
BBa_K157004 Version 1 (Component)
Fluoresceine-A-binding

pRecA
BBa_K093000 Version 1 (Component)
pRecA with LexA binding site

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K102906
BBa_K102906 Version 1 (Component)
TA8 gate from synthetic algorithm v1.1

BBa_K102907
BBa_K102907 Version 1 (Component)
TA9 gate from synthetic algorithm v1.1

BBa_K102908
BBa_K102908 Version 1 (Component)
TA10 gate from synthetic algorithm v1.1

BBa_K102909
BBa_K102909 Version 1 (Component)
TA11 gate from synthetic algorithm v1.1

BBa_K102910
BBa_K102910 Version 1 (Component)
TA12 gate from synthetic algorithm v1.1

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard