Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

lux pL
BBa_R0063 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pL)<br>

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

xis
BBa_K112200 Version 1 (Component)
{xis!} from bacteriophage lambda; assembly standard 21

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

lux pR
BBa_R1062 Version 1 (Component)
Promoter, Standard (luxR and HSL regulated -- lux pR)<br>

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

xis
BBa_K112001 Version 1 (Component)
Xis from bacteriophage lambda, assembly standard 21

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

xis domain
BBa_K112203 Version 1 (Component)
{< xis >} The bacteriophage lambda xis gene without start or stop codons; assembly standard 21

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

BBa_P0451
BBa_P0451 Version 1 (Component)
PoPS -> cI (lambda) [S0153]

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_K1159993
BBa_K1159993 Version 1 (Component)
25/25 Assembly Scar

cI 434
BBa_R1052 Version 1 (Component)
Promoter, Standard (434 cI regulated)<br>

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

cI HK022
BBa_R1050 Version 1 (Component)
Promoter, Standard (HK022 cI regulated) <br>

BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

BBa_I15008
BBa_I15008 Version 1 (Component)
heme oxygenase (ho1) from Synechocystis