Cas9
Cas9 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

mCherry
BBa_J06505 Version 1 (Component)
monomeric RFP optimized for bacteria

BBa_K1774001
BBa_K1774001 Version 1 (Component)
Cas9 (optimized for expression in E. coli)

BBa_M1716
BBa_M1716 Version 1 (Component)
Hepcidin Optimized for E. coli

BBa_I746200
BBa_I746200 Version 1 (Component)
FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.

Intein
BBa_K300004 Version 1 (Component)
Engineered pH-inducible intein (codon optimized for E. coli) - internal domain

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

sacB
BBa_K322921 Version 1 (Component)
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.

yfp
BBa_K165005 Version 1 (Component)
Venus YFP, yeast optimized for fusion

BBa_K953004
BBa_K953004 Version 1 (Component)
Hemeoxygenase with RBS (E. coli Codon Optimised)

BBa_K1027001
BBa_K1027001 Version 1 (Component)
Delta 9 Desaturase, optimised for expression in Escherichia coli

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

Cse1
BBa_K1218003 Version 1 (Component)
CRISPR CasA E. coli (Modern)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_K953002
BBa_K953002 Version 1 (Component)
Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)

BBa_K1321338
BBa_K1321338 Version 1 (Component)
T7 Expression vector

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_K1027002
BBa_K1027002 Version 1 (Component)
Delta 12 Desaturase, optimised for expression in Escherichia coli

adhB
BBa_K173017 Version 1 (Component)
alcohol dehydrogenase II from Z. mobilis (codon optimized for E. coli)

dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

BBa_K325210
BBa_K325210 Version 1 (Component)
Red Firefly Luciferase and LRE<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

pdc
BBa_K173016 Version 1 (Component)
pyruvate decarboxylase from Z. mobilis (codon optimized for E. coli)

BBa_K1319004
BBa_K1319004 Version 1 (Component)
TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli

mCherry
BBa_K165004 Version 1 (Component)
mCherry, yeast optimized for fusion