Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

GAL1
BBa_J63006 Version 1 (Component)
yeast GAL1 promoter

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_I746916
BBa_I746916 Version 1 (Component)
superfolder GFP coding sequence

mCherry
BBa_E2060 Version 1 (Component)
derivative of mRFP1, yeast-optimized

BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

mRFP1
BBa_E2050 Version 1 (Component)
derivative of mRFP1, yeast-optimized

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

lacZ-alpha
BBa_I732006 Version 1 (Component)
lacZ alpha fragment

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

yfp
BBa_K165005 Version 1 (Component)
Venus YFP, yeast optimized for fusion

BBa_K801012
BBa_K801012 Version 1 (Component)
ADH1 yeast terminator

BBa_K953004
BBa_K953004 Version 1 (Component)
Hemeoxygenase with RBS (E. coli Codon Optimised)

BBa_E2030
BBa_E2030 Version 1 (Component)
enhanced version of EYFP, yeast-optimized

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_K1641003
BBa_K1641003 Version 1 (Component)
Recombinase Vika, E. coli and yeast coding optimized

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

Intein
BBa_K300004 Version 1 (Component)
Engineered pH-inducible intein (codon optimized for E. coli) - internal domain

BBa_K953002
BBa_K953002 Version 1 (Component)
Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_K118002
BBa_K118002 Version 1 (Component)
crtB coding sequence encoding phytoene synthase

BBa_K118003
BBa_K118003 Version 1 (Component)
crtI coding sequence encoding phytoene dehydrogenase

mCherry
BBa_K165004 Version 1 (Component)
mCherry, yeast optimized for fusion

BBa_K953000
BBa_K953000 Version 1 (Component)
Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)

amilCP
BBa_K592009 Version 1 (Component)
amilCP, blue chromoprotein

BBa_M1716
BBa_M1716 Version 1 (Component)
Hepcidin Optimized for E. coli

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

BBa_K165010
BBa_K165010 Version 1 (Component)
ADH1 yeast transcription terminator

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

BBa_K801080
BBa_K801080 Version 1 (Component)
Prepro-Thaumatin, yeast codon optimized

BBa_Z0301
BBa_Z0301 Version 1 (Component)
Venus YFP - E. coli codon optimized