Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

BBa_K2186000
BBa_K2186000 Version 1 (Component)
gRNA targeting TolC in E. Coli BL21(DE3)

BBa_K2186001
BBa_K2186001 Version 1 (Component)
gRNA targeting AcrA in E. Coli BL21(DE3)

BBa_K2186002
BBa_K2186002 Version 1 (Component)
gRNA targeting AcrB in E. Coli BL21(DE3)

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

BBa_K554009
BBa_K554009 Version 1 (Component)
TolC

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K1393003
BBa_K1393003 Version 1 (Component)
outer membrane porin protein C from Escherichia coli BL21(DE3)

sacB
BBa_K322921 Version 1 (Component)
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

BBa_K1900003
BBa_K1900003 Version 1 (Component)
E. coli tolC

BBa_K1683003
BBa_K1683003 Version 1 (Component)
E. coli tolC signaling sequence + Proteus mirabilis tolC

BBa_K1683001
BBa_K1683001 Version 1 (Component)
E. coli tolC signaling sequence + Yersenia Pestis tolC

BBa_J100231
BBa_J100231 Version 1 (Component)
tolC Constitutive Promoter in E. coli

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_I746200
BBa_I746200 Version 1 (Component)
FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.

BBa_K228001
BBa_K228001 Version 1 (Component)
SupD-tRNA

BBa_K1393002
BBa_K1393002 Version 1 (Component)
cyn operon in Escherichia coli BL21(DE3) (cynR+cynT+cynS)

BBa_K115002
BBa_K115002 Version 1 (Component)
RNA thermometer (FourU)

BBa_K912001
BBa_K912001 Version 1 (Component)
E. coli phytase (appa phytase)

BBa_K1900002
BBa_K1900002 Version 1 (Component)
pBAD+strong RBS+E. coli tolC signal sequence+E. coli tolC

BBa_K1683005
BBa_K1683005 Version 1 (Component)
E. coli tolC signaling sequence + Pseudomonas aeruginosa oprM

BBa_K115001
BBa_K115001 Version 1 (Component)
RNA thermometer (ROSE)

TEVsite
BBa_J18918 Version 1 (Component)
TEV cleavage site (E. coli)

BBa_I2032
BBa_I2032 Version 1 (Component)
T7 RNA polymerase

BBa_K953004
BBa_K953004 Version 1 (Component)
Hemeoxygenase with RBS (E. coli Codon Optimised)

BBa_K1172901
BBa_K1172901 Version 1 (Component)
Alanine racemase from ''E. coli''

BBa_K863006
BBa_K863006 Version 1 (Component)
ecol laccase from E. coli

BBa_K1683002
BBa_K1683002 Version 1 (Component)
pBAD+Strong RBS+E. coli tolC signal sequence+P. mirabilis tolC

BBa_K1683000
BBa_K1683000 Version 1 (Component)
pBAD+Strong RBS+E. coli tolC signal sequence+Y. pestis tolC

MarA
BBa_K1230000 Version 1 (Component)
AcrAB-TolC multidrug efflux transport system activator from E. coli

BBa_J70024
BBa_J70024 Version 1 (Component)
TetM gene, recoded compromise E. coli & Mesoplasma florum

ssrA(DAS+4
BBa_K1075020 Version 1 (Component)
E. coli ssrA(DAS+4)

BBa_K1900000
BBa_K1900000 Version 1 (Component)
pBAD+strong RBS+E. coli tolC signal sequence+K. pneumoniae tolC

BBa_K1900001
BBa_K1900001 Version 1 (Component)
pBAD+strong RBS+E. coli tolC signal sequence+S. marcescens tolC

BBa_M1716
BBa_M1716 Version 1 (Component)
Hepcidin Optimized for E. coli

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard