BBa_K1933003
BBa_K1933003 Version 1 (Component)
constitutive expression of CBDclos fused to BclA with 6xHis tag

BBa_M45134
BBa_M45134 Version 1 (Component)
Uranium reduction from VI valent state to IV valent state

BBa_I758601
BBa_I758601 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

Amphiphili
BBa_K1655002 Version 1 (Component)
Amphiphilic proteins can be used to form intracellular micelles or vesicles.

BBa_K1321117
BBa_K1321117 Version 1 (Component)
CBDcenA-linker fused to Phytochelatin (PC) EC20 with LacI promoter

BBa_K1321107
BBa_K1321107 Version 1 (Component)
CBDcen-linker fused to Phytochelatin (PC) EC20 with T7 promoter

BBa_K1113002
BBa_K1113002 Version 1 (Component)
Targeting sequence for the delivery of GFP to the Carboxysome

BBa_K935005
BBa_K935005 Version 1 (Component)
Two E. coli ArsR generators coupled to pArs-ArsR-RFP

BBa_M50047
BBa_M50047 Version 1 (Component)
Linear DNA to be used with HIV I Integrase pD649

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K737039
BBa_K737039 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by aTc

BBa_S04147
BBa_S04147 Version 1 (Component)
The Partial Butanol Operon (5 inserts from I725021 to I725025)

BBa_K737038
BBa_K737038 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by IPTG

BBa_K1321350
BBa_K1321350 Version 1 (Component)
CBDcenA with linker fused to sfGFP in Freiburg format (RFC 25)

BBa_K1588007
BBa_K1588007 Version 1 (Component)
Protein with epitopes from dengue virus serotype 2 to generate immune response

BBa_K1588006
BBa_K1588006 Version 1 (Component)
Protein with epitopes from dengue virus serotype 1 to generate immune response

BBa_K1588008
BBa_K1588008 Version 1 (Component)
Protein with epitopes from dengue virus serotype 3 to generate immune response

BBa_K1588009
BBa_K1588009 Version 1 (Component)
Protein with epitopes from dengue virus serotype 4 to generate immune response

BBa_J45013
BBa_J45013 Version 1 (Component)
PAL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid

BBa_J45011
BBa_J45011 Version 1 (Component)
PAL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid

BBa_I20249
BBa_I20249 Version 1 (Component)
Device used to characterize Ribosome Binding Sites through measurement of GFP expression

BBa_J70306
BBa_J70306 Version 1 (Component)
Similar to S03621, except PsrI sites (some portions of B0010, B0012) removed

BBa_I20248
BBa_I20248 Version 1 (Component)
Device used to characterize Ribosome Binding Sites through measurement of GFP expression

BBa_I20250
BBa_I20250 Version 1 (Component)
Device used to characterize Ribosome Binding Sites through measurement of RFP expression

BBa_I20251
BBa_I20251 Version 1 (Component)
Device used to characterize Ribosome Binding Sites through measurement of RFP expression

BBa_K1113400
BBa_K1113400 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome.

BBa_K1986002
BBa_K1986002 Version 1 (Component)
Is an homologuous of reinhardi and works to give an lipidic expresion

BBa_K1113410
BBa_K1113410 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome.

PatgF1GFP
BBa_K844015 Version 1 (Component)
lac/IPTG inducible Spider Silk 1x "F" Subunit fused to GFP

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_K1615065
BBa_K1615065 Version 1 (Component)
Linker-CBDcipA-linker fused to Heroin Esterase driven by LacI promoter

BBa_K1615013
BBa_K1615013 Version 1 (Component)
MorA fused to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K1615035
BBa_K1615035 Version 1 (Component)
MaoA fused to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K1615102
BBa_K1615102 Version 1 (Component)
mRFP used to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K1615066
BBa_K1615066 Version 1 (Component)
Heroin Esterase fused to Linker-CBDcipA-linker driven by LacI promoter

BBa_K1615080
BBa_K1615080 Version 1 (Component)
TVEL5 fused to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K322710
BBa_K322710 Version 1 (Component)
Marker construct which can be used to target tnaA in E.coli.

BBa_K1932006
BBa_K1932006 Version 1 (Component)
This device is constructed to express TAT-apoptin fused with sec2.

BBa_K1615088
BBa_K1615088 Version 1 (Component)
TVEL5 laccase fused to Linker-CBDcipA-linker driven by LacI promoter

BBa_K1890002
BBa_K1890002 Version 1 (Component)
Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS

BBa_K1833011
BBa_K1833011 Version 1 (Component)
lac promoter -> Zinc finger 225 binding domain fused to immunogenic MUC1 peptide

BBa_K1698003
BBa_K1698003 Version 1 (Component)
BaseHunter : Activated to become detector for 32bp target sequence on SRY Gene

BBa_K1698004
BBa_K1698004 Version 1 (Component)
BaseHunter : Activated to become detector for 24bp target sequence on Mycobacterium genomes

BBa_K1392910
BBa_K1392910 Version 1 (Component)
with L+ arabinose promoter, caterchol 2,3-dioxygenase activate to degration of catechol.

BBa_K1657005
BBa_K1657005 Version 1 (Component)
It is called BAG. It have the resistance to glyphosate and glufosinate

BBa_K1657007
BBa_K1657007 Version 1 (Component)
It is called GAB. It have the resistance to glyphosate and glufosinate