LipABBa_K836000 Version 1 (Component)lipA from B. cepacia (codon usage optimized for E. coli)
DGATBBa_K836001 Version 1 (Component)O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus)
DGATBBa_K836002 Version 1 (Component)O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used
lipABBa_K836003 Version 1 (Component)lipA from B. cepacia (codon usage optimized for E. coli)
BBa_K750005BBa_K750005 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)
BBa_K750006BBa_K750006 Version 1 (Component)LuxR expression device activated by arabinose
BBa_K737038BBa_K737038 Version 1 (Component)galK::GFP generator ligated to sRNA device controlled by IPTG
BBa_K737039BBa_K737039 Version 1 (Component)galK::GFP generator ligated to sRNA device controlled by aTc
BBa_K737040BBa_K737040 Version 1 (Component)galK::GFP generator ligated to TetR generator and constitutive sRNA device
BBa_K737041BBa_K737041 Version 1 (Component)galK::GFP generator ligated to LacI generator and constitutive sRNA device
gpSBBa_K836004 Version 1 (Component)Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)
BM-CFPBBa_K774004 Version 1 (Component)Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420
BM-RFPBBa_K774005 Version 1 (Component)Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B
MB-CFPBBa_K774006 Version 1 (Component)Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420
MB-RFPBBa_K774007 Version 1 (Component)Mammalian-Bacterial promoter with RFP reporter: CArG promoter sequence + BBaK216005 + BBa_K08101
BBa_K782019BBa_K782019 Version 1 (Component)10x[TALA+TALB] operator_CMV promoter_fLuciferase
BBa_K782020BBa_K782020 Version 1 (Component)10x[TALA] operator_CMV promoter_fLuciferase
BBa_K782021BBa_K782021 Version 1 (Component)10x[TALB] operator_CMV promoter_fLuciferase
BBa_K782022BBa_K782022 Version 1 (Component)12x[TALD] operator_CMV promoter_fLuciferase
BBa_K782023BBa_K782023 Version 1 (Component)12x[NicTAL] operator_CMV promoter_fLuciferase
BBa_K113015BBa_K113015 Version 1 (Component)bidirectional intact T7 promoters->GFP/RFP
BBa_K836005BBa_K836005 Version 1 (Component)Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)
gpSBBa_K836006 Version 1 (Component)Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
nitRBBa_K836007 Version 1 (Component)Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Cello E. Coli PartsEco1C1G1T1_collection Version 1 (Collection)These are the Cello parts for E. Coli circuits
BBa_K098991BBa_K098991 Version 1 (Component)cI promoter with GFP reporter
BBa_S04138BBa_S04138 Version 1 (Component)cI QPI with GFP reporter system
BBa_K098988BBa_K098988 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and high promoter
BBa_K098987BBa_K098987 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and low promoter
BBa_K098982BBa_K098982 Version 1 (Component)lac inducible system with low promoter and GFP reporter
BBa_K782032BBa_K782032 Version 1 (Component)10x[TALA+TALB] operator_minimal promoter_fLuciferase
BBa_K782033BBa_K782033 Version 1 (Component)10x[TALA] operator_minimal promoter_fLuciferase
Tm-Split-LBBa_K157020 Version 1 (Component)Tm-Split-Luciferase1/2
rTT+pRBBa_K093003 Version 1 (Component)Convergent Promoter System: Forward Module: rTT+lambda pR
Q04510+RFPBBa_K131018 Version 1 (Component)Intermediate for the Response circuit
BBa_K101016BBa_K101016 Version 1 (Component)Dually repressed promoter with sites for TetR and P22MNT binding
BBa_J69522BBa_J69522 Version 1 (Component)tetR-repressible promoter coupled with mCherry Reporter
Tm-SplitluBBa_K157021 Version 1 (Component)Tm-Splitluciferase2/2
SP-AntiNIPBBa_K157030 Version 1 (Component)SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
SP-AntiNIPBBa_K157031 Version 1 (Component)SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
SP-LipocalBBa_K157038 Version 1 (Component)SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2
SP-LipocalBBa_K157039 Version 1 (Component)SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
BBa_K125810BBa_K125810 Version 1 (Component)slr2016 signal sequence + GFP fusion for secretion of GFP
OriTRBBa_J01003 Version 1 (Component)OriT-R (Origin of transfer for the R-plasmid nic region)
pBSEP1BBa_K090401 Version 1 (Component)Gram-positive Shuttle Vector for Episomal Expression
BBa_K086001BBa_K086001 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter
BBa_K086003BBa_K086003 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter