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Showing 1851 - 1900 of 1924 result(s)
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Public
BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato
Public
Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
Public
BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway
Public
BBa_I716212
BBa_I716212 Version 1 (Component)
Cre (GTG start)
Public
BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein
Public
BBa_K2150029
BBa_K2150029 Version 1 (Component)
pCon+RBS+tetR+DT+pTet+RBS+T7RNAP+Ter+RBS+pT7+tetX-GFP(fusion protein)+DT
Public
BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene
Public
BBa_K187416
BBa_K187416 Version 1 (Component)
trpE in pAB
Public
Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')
Public
BBa_K327013
BBa_K327013 Version 1 (Component)
The initiator
Public
BBa_J3106
BBa_J3106 Version 1 (Component)
pBad-HixC-TetB-RBS<sub>rev</sub>-HixC-TT-RE
Public
BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock
Public
BBa_K594015
BBa_K594015 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP
Public
BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock
Public
BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome
Public
BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
Public
BBa_I715065
BBa_I715065 Version 1 (Component)
Burnt Pancake Tester with RE and backwards Tet resistance
Public
Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Public
BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter
Public
BBa_K1361003
BBa_K1361003 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C
Public
BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
Public
BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
Public
BBa_K1361001
BBa_K1361001 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively weak expression of CsgA,C
Public
BBa_J58009
BBa_J58009 Version 1 (Component)
Fusion protein Trg-EnvZ
Public
BBa_K2044000
BBa_K2044000 Version 1 (Component)
Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8
Public
BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins
Public
BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
Public
BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI
Public
BBa_J44008
BBa_J44008 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF
Public
BBa_J44006
BBa_J44006 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE
Public
BBa_J44007
BBa_J44007 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF
Public
BBa_J44005
BBa_J44005 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE
Public
BBa_K1947028
BBa_K1947028 Version 1 (Component)
Improve the protein purification.
Public
BBa_K1947027
BBa_K1947027 Version 1 (Component)
Improve the protein purification.
Public
BBa_K1947026
BBa_K1947026 Version 1 (Component)
Improve the protein purification.
Public
BBa_K2036028
BBa_K2036028 Version 1 (Component)
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase
Public
BBa_K2036027
BBa_K2036027 Version 1 (Component)
pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
Public
BBa_K886002
BBa_K886002 Version 1 (Component)
Recombination Device composed by Cre-lox71
Public
BBa_K2036024
BBa_K2036024 Version 1 (Component)
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase
Public
BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.
Public
BBa_S03596
BBa_S03596 Version 1 (Component)
AraCPC<sub>rev</sub>-TT : pBad-HixC-RBS-TetF-HixC-TT-RE
Public
BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.
Public
Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit
Public
BBa_J70652
BBa_J70652 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te
Public
BBa_K1351017
BBa_K1351017 Version 1 (Component)
SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>
Public
BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.
Public
BBa_I715069
BBa_I715069 Version 1 (Component)
Test part to see if RE and pLac +hixc can be expressed with GFP
Public
BBa_J70653
BBa_J70653 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te
Showing 1851 - 1900 of 1924 result(s)
Previous 33 34 35 36 37 38 39 Next