BBa_K113015BBa_K113015 Version 1 (Component)bidirectional intact T7 promoters->GFP/RFP
BBa_K105027BBa_K105027 Version 1 (Component)cyc100 minimal promoter
BBa_K113016BBa_K113016 Version 1 (Component)overlapping T7 promoter->GFP/RFP
BBa_K113017BBa_K113017 Version 1 (Component)more overlapping T7 promoter->GFP/RFP
BBa_K105028BBa_K105028 Version 1 (Component)cyc70 minimal promoter
BBa_K105029BBa_K105029 Version 1 (Component)cyc43 minimal promoter
BBa_K105030BBa_K105030 Version 1 (Component)cyc28 minimal promoter
BBa_K105031BBa_K105031 Version 1 (Component)cyc16 minimal promoter
BBa_K113021BBa_K113021 Version 1 (Component)Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter
cI 434BBa_R0052 Version 1 (Component)Promoter (434 cI regulated)
BBa_K113012BBa_K113012 Version 1 (Component)weaken overlapping T7 promoter
BBa_K113018BBa_K113018 Version 1 (Component)weaken overlapping T7 promoter->GFP/RFP
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
BBa_K098999BBa_K098999 Version 1 (Component)mtrB with RBS
BBa_K098998BBa_K098998 Version 1 (Component)cIts coding region with rbs
BBa_K098996BBa_K098996 Version 1 (Component)mtrB with terminator
BBa_K098994BBa_K098994 Version 1 (Component)mtrB with RBS and terminator
BBa_K098995BBa_K098995 Version 1 (Component)heat sensitive cI QPI with high promoter
BBa_K098993BBa_K098993 Version 1 (Component)heat sensitive cI QPI with low promoter
BBa_K098991BBa_K098991 Version 1 (Component)cI promoter with GFP reporter
BBa_S04138BBa_S04138 Version 1 (Component)cI QPI with GFP reporter system
BBa_K098988BBa_K098988 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and high promoter
BBa_K098987BBa_K098987 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and low promoter
BBa_K098982BBa_K098982 Version 1 (Component)lac inducible system with low promoter and GFP reporter
pRecABBa_K093000 Version 1 (Component)pRecA with LexA binding site
BBa_J24815BBa_J24815 Version 1 (Component)J23101 promoter with Renilla gene attached
BBa_J24816BBa_J24816 Version 1 (Component)J23101 promoter with Luc gene attached
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
rTT+pRBBa_K093003 Version 1 (Component)Convergent Promoter System: Forward Module: rTT+lambda pR
BBa_K090501BBa_K090501 Version 1 (Component)Gram-Positive IPTG-Inducible Promoter
BBa_K090502BBa_K090502 Version 1 (Component)Gram-Positive Xylose-Inducible Promoter
BBa_K101016BBa_K101016 Version 1 (Component)Dually repressed promoter with sites for TetR and P22MNT binding
BBa_K143071BBa_K143071 Version 1 (Component)AmyE integratable PoPS generator (P43-gsiB) (with CmR)
BBa_K143072BBa_K143072 Version 1 (Component)AmyE integratable PoPS generator (P43-spoVG) (with CmR)
BBa_K143073BBa_K143073 Version 1 (Component)AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)
BBa_K143074BBa_K143074 Version 1 (Component)AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)
BBa_J69522BBa_J69522 Version 1 (Component)tetR-repressible promoter coupled with mCherry Reporter
BBa_K090503BBa_K090503 Version 1 (Component)Gram-Positive General Constitutive Promoter
BBa_K090504BBa_K090504 Version 1 (Component)Gram-Positive Strong Constitutive Promoter
BBa_K143075BBa_K143075 Version 1 (Component)B. subtilis AmyE locus GFP output promoter and RBS tester
BBa_K143076BBa_K143076 Version 1 (Component)B. subtilis AmyE locus RFP output promoter and RBS tester
rPlac+TTBBa_K093004 Version 1 (Component)Convergent Promoter System: Reverse Module: rPlac+TT
BBa_K125110BBa_K125110 Version 1 (Component)nir promoter + rbs (0.6)
MioCBBa_K101017 Version 1 (Component)MioC Promoter (DNAa-Repressed Promoter)
B-H-R plasBBa_K125020 Version 1 (Component)lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT
BBa_K086000BBa_K086000 Version 1 (Component)unmodified Lutz-Bujard LacO promoter - YFP
BBa_K086017BBa_K086017 Version 1 (Component)unmodified Lutz-Bujard LacO promoter
BBa_K090506BBa_K090506 Version 1 (Component)''Bacillus subtilis'' weak RBS
BBa_K122016BBa_K122016 Version 1 (Component)4CL:STS with CYC1 terminator.
RBS-RFPBBa_K093005 Version 1 (Component)RFP with RBS