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Public
SEGA
SEGA_collection Version 1 (Unknown)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by ?-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic ?control elements? that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Public
BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency
Public
BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7
Public
BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB
Public
BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)
Public
p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter
Public
GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
Public
LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)
Public
BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss
Public
BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030
Public
tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)
Public
lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)
Public
lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)
Public
mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Public
BBa_J23100
BBa_J23100 Version 1 (Component)
constitutive promoter family member
Public
scar
BBa_J176041 Version 1 (Component)
Silver scar
Public
lux pR
BBa_R0062 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pR)
Public
cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)
Public
BBa_J23119
BBa_J23119 Version 1 (Component)
constitutive promoter family member
Public
BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device
Public
pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter
Public
luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)
Public
eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP
Public
cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)
Public
BBa_J23101
BBa_J23101 Version 1 (Component)
constitutive promoter family member
Public
P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ
Public
BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030
Public
mCh
BBa_J176005 Version 1 (Component)
mCh
Public
luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL
Public
BBa_J23106
BBa_J23106 Version 1 (Component)
constitutive promoter family member
Public
pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid
Public
BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member
Public
BBa_I13453
BBa_I13453 Version 1 (Component)
Pbad promoter
Public
Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette
Public
BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)
Public
pT7
BBa_I719005 Version 1 (Component)
T7 Promoter
Public
BBa_B1001
BBa_B1001 Version 1 (Component)
Terminator (artifical, small, %T~=90)
Public
GFP report
BBa_E0240 Version 1 (Component)
GFP generator
Public
YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP
Public
BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS
Public
BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)
Public
Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence
Public
ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP
Public
BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030
Public
BBa_J23110
BBa_J23110 Version 1 (Component)
constitutive promoter family member
Public
BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member
Public
BBa_B1006
BBa_B1006 Version 1 (Component)
Terminator (artificial, large, %T~>90)
Public
BBa_I13507
BBa_I13507 Version 1 (Component)
Screening plasmid intermediate
Public
BBa_J56012
BBa_J56012 Version 1 (Component)
Invertible sequence of dna includes Ptrc promoter
Public
BBa_K1847014
BBa_K1847014 Version 1 (Component)
placUV5-lacO
Showing 1 - 50 of 10000 result(s)
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